Leptin from mouse
SIGMA/L3772 - ≥98% (SDS-PAGE), recombinant, expressed in E. coli, lyophilized powder
Synonym: OB
CAS Number: 181030-10-4
MDL Number: MFCD01094206
Product Type: Chemical
Purity The purity of Leptin mouse (Cat. No. L3772) was assessed on SDS-PAGE stained with InstantBlue™ (Cat. No. ISB1L).
Bioassay The biological activity of Leptin mouse (Cat. No. L3772) was measured in a cell proliferation assay using Baf3-OB-Rb cells. The EC50 is defined as the effective concentration that elicits a 50% increase in cell growth in a cell-based bioassay.
| assay | ≥98% (SDS-PAGE) |
| biological source | mouse |
| form | lyophilized powder |
| impurities | ≤1.0 EU/μg endotoxin |
| InChI | 1S/C87H138N22O28S2/c1-41( |
| InChI key | NRYBAZVQPHGZNS-ZSOCWYAHSA |
| mol wt | predicted mol wt ~16 kDa |
| potency | ≤1.0 ng/mL ED50 |
| Quality Level | 200  |
| recombinant | expressed in E. coli |
| shipped in | ambient |
| SMILES string | CC(C)CC(C(=NC(CCC(=N)O)C( |
| sterility | non-sterile |
| storage condition | OK to freeze |
| storage temp. | −20°C |
| technique(s) | cell based assay: suitable |
| UniProt accession no. | P41160  |
| Analysis Note: | The biological activity is measured by its ability to induce proliferation of leptin-dependent human OB-R transfected murine BAF3 cells. |
| Application: | Leptin from mouse has been used: • to check the effect of leptin on ovulation for treating infertility in obese mice • to check the effect of leptin on MGARP (mitochondria-localized glutamic acid-rich protein) expression in testis and follicular granular cells of mice • to study the morphometrical changes in mouse testicular tissue upon long term exposure to leptin |
| Biochem/physiol Actions: | Hormone produced primarily in adipocytes; primary site of action appears to be on neurons in the hypothalamus that are involved in regulating energy balance, appetite, and body weight. |
| Biochem/physiol Actions: | Leptin is a hormone produced primarily in adipocytes, although leptin mRNA has also been identified in placenta and fetal tissues, gastric tissue and liver. Its primary site of action appears to be on neurons in the hypothalamus that are involved in regulating energy balance, appetite, and body weight. Leptin increases the production of nitric oxide in endothelial cells and stimulates angiogenesis in vitro and in vivo. Human and mouse leptin share ~84% sequence identity. |
| Biochem/physiol Actions: | Leptin, an essential cytokine, serves as a signaling molecule in the reproductive system. It modulates placental functions through autocrine or paracrine signaling. Particularly in the early stages of pregnancy, leptin plays a vital role in modulating key processes including cell proliferation, protein synthesis, invasion, and apoptosis within placental cells. Deficiency of leptin may lead to the development of severe obesity and abnormalities in hematopoiesis, immunity, angiogenesis, bone formation, blood pressure regulation and reproduction. Absence of leptin in mice causes severe obesity and hypothermia at temperature below thermoneutrality. It also controls feeding behavior. Leptin signaling is crucial for the bone formation. |
| General description: | Research area: Cell signaling Leptin (Ob) is a non-glycosylated polypeptide hormone. It is produced primarily in adipocytes and the placenta. The structure of leptin closely resembles members of the long-chain helical cytokine family. It is composed of four antiparallel α-helices interconnected by two long crossover links and one short loop. These structural elements are arranged in a left-handed helical bundle formation, resulting in a unique two-layer packing configuration. |
| Physical form: | Lyophilized from a 0.2 μm filtered solution with 0.0045 mM NaHCO3 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥98% (SDS-PAGE) |
| Storage Temp. | −20°C |
| UNSPSC | 51111800 |