Prolactin human
SIGMA/L4021 - recombinant, expressed in E. coli, lyophilized powder, BioReagent, suitable for cell culture, >97% (SDS-PAGE)
Synonym: hPRL; LTH; Lactogenic Hormone; Luteotropic Hormone; PRL
CAS Number: 9002-62-4
MDL Number: MFCD00131553
Product Type: Chemical
SDS-PAGE Prolactin human (Cat. No. L4021) was assessed on SDS-PAGE.
Bioassay EC50 is defined as the effective concentration of Prolactin that elicits a 50% attachment factor in cell based bioassay.
| assay | >97% (SDS-PAGE) |
| biological source | Escherichia coli |
| description | 200 amino acid residue methionyl form of mature, recombinant human prolactin (amino acid residues 29-227). |
| form | lyophilized powder |
| impurities | endotoxin, tested |
| mol wt | predicted mol wt ~24 kDa |
| potency | 0.030-0.300 ng/mL EC50 |
| product line | BioReagent |
| Quality Level | 200  |
| recombinant | expressed in E. coli |
| shipped in | ambient |
| solubility | water: 0.5000 mL, clear, colorless |
| storage temp. | −20°C |
| technique(s) | cell culture | mammalian: suitable |
| UniProt accession no. | P01236  |
| Analysis Note: | The biological activity is assayed by proliferation of the rat lymphoma cell line, Nb2-11. |
| Application: | Use in cell culture applications to study prolactin regulation of cell signaling and gene expression. |
| Biochem/physiol Actions: | Neuroendocrine hormone; belongs to the cytokine hematopoietic receptor family; induces lactation; inhibits secretion of gonadotropins; release is inhibited by dopamine. |
| Biochem/physiol Actions: | Prolactin is a neuroendocrine hormone. The prolactin receptor is a transmembrane glycoprotein that belongs to the cytokine hematopoietic receptor family. A large number of cells and organs express the receptor, including B cells, T cells, macrophages, monocytes, and neutrophils. Prolactin signal transduction involves the JAK/STAT families and the src kinase family. Induces lactation; inhibits secretion of gonadotropins; release is inhibited by dopamine. |
| Other Notes: | Contains an N-terminal methionine not present in the natural product. |
| Physical form: | Lyophilized from a solution in 10 mM phosphate and 50 mM sodium chloride, pH 8.0. |
| Preparation Note: | It is recommended that sterile 4 mM HCl containing 1 mg/mL bovine serum albumin be added to the vial to prepare a stock solution of no less than 100 μg/mL. |
| Symbol |  GHS08 |
| Signal word | Danger |
| Hazard statements | H360 |
| Precautionary statements | P201 - P308 + P313 |
| Hazard Codes | T |
| Risk Statements | 60 |
| Safety Statements | 53-45 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | >97% (SDS-PAGE) |
| Storage Temp. | −20°C |
| UNSPSC | 51111800 |