Lectin from Phaseolus vulgaris (red kidney bean)
SIGMA/L4144 - Leucoagglutinin PHA-L, lyophilized powder, BioReagent, suitable for cell culture
Synonym: Phaseolus vulgaris agglutinin; PHA
EC Number: 232-718-7
Product Type: Chemical
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| form | lyophilized powder |
| impurities | salt, free |
| potency | erythroagglutination activity (Does not agglutinate a 2% suspension of human erythrocytes at 250 μg lectin/ml.) |
| ≤1.0 μg per mL leucoagglutination activity | |
| ≤5 μg per mL mitogenic activity (Bromdeoxyuridine incorporation in human peripheral blood mononuclear cells) | |
| product line | BioReagent |
| Quality Level | 200  |
| solubility | DPBS: 5 mg/mL, slightly hazy, colorless |
| storage temp. | 2-8°C |
| suitability | leucocyte agglutination activity tested |
| technique(s) | cell culture | mammalian: suitable |
| Analysis Note: | Agglutination activity is expressed in μg/ml and is determined from serial dilutions of a 1 mg/ml solution. This activity is the lowest concentration to agglutinate a suspension of either human erythrocytes (2% in phosphate buffered saline, pH 6.8) or human leukocytes (107 per mL in saline) after 1 hr incubation at 25 °C. |
| Application: | Lectin from Phaseolus vulgaris (red kidney bean) has been used: • it has been used as a positive control to treat cells • to stimulate cell culture for the analysis of lymphocyte proliferation • to stimulate T cells |
| Biochem/physiol Actions: | Lectin agglutinates cells and precipitates polysaccharides and glycoproteins. It is associated with toxic allergens and hemagglutinins. Lectin exhibits anti-cancer, anti-human immunodeficiency virus (anti-HIV) and anti-microbial infection properties. Lectin inhibits mucosal atrophy, lowers type 2 diabetes and obesity, enhances nutrient absorption and regulates drug targeting. |
| Biochem/physiol Actions: | PHA consists of two molecular species, an erythroagglutinin (PHA-E) which has low mitogenic activity and high erythroagglutinin activity, and leucoagglutinin (PHA-L) which has high mitogenic and leucoagglutinating activity, but very low erythroagglutinating activity. PHA-E is not blood group specific, but agglutination can be inhibited by certain oligosaccharides. PHA-P is the protein form of PHA prior to separation and purification of erythroagglutinin and leucoagglutinin. PHA-M is the mucoprotein form. Conjugates are prepared from the corresponding purified lectins. |
| General description: | Lectins are carbohydrate-binding proteins, which contains four subunits with a molecular weight of 125 kDa. Leucoagglutinin PHA-L is a plant lectin, which is transported by the neurons in the central nervous system. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352202 |