α-Lactalbumin from bovine milk
SIGMA/L4385 - Marker for non-denaturing PAGE
CAS Number: 9051-29-0
EC Number: 235-747-3
MDL Number: MFCD00162025
Product Type: Chemical
This image is provided for informative purposes only and does not represent an actual product label. It should not be used as a substitute for official product labeling.
| biological source | bovine milk |
| concentration | 0.9—1.4 mg per vial protein (biuret) |
| form | lyophilized powder |
| mol wt | ~14.2 kDa |
| Quality Level | 200  |
| storage temp. | −20°C |
| technique(s) | microbiological culture: suitable |
| UniProt accession no. | P00711  |
| Application: | α-Lactalbumin was used in the isolation and analysis of restriction endonuclease digestive patterns of chromosomal DNA from Mycobacterium paratuberculosis and other Mycobacterium species. It was also used in a study to test if the neuroendocrine protein 7B2 suppresses the aggregation of neurodegenerative disease-related proteins. |
| Biochem/physiol Actions: | Alters the substrate specificity of galactosyltransferase to increase the rate of lactose formation; the complex of galactosyltransferase and α-lactalbumin is called lactose synthase. |
| Biochem/physiol Actions: | Alters the substrate specificity of galactosyltransferase to increase the rate of lactose formation; the complex of galactosyltransferase and α-lactalbumin is called lactose synthase. Site-directed mutagenesis of Asp87 or Asp88 to Ala completely abolishes the strong calcium binding affinity and reduces the stimulation of lactose synthase to <3.5% of the maximal rate. |
| Biochem/physiol Actions: | α-Lactalbumin is the cheif protein in human milk. It consists of a single polypeptide chain with 8 cysteines which form disulfide bridges. α-Lactalbumin binds several metal ions, including calcium, which is thought to play a role in the regeneration of native α-lactalbumin from the reduced, denatured form. α-Lactalbumin also has a distinct zinc binding site that is thought to play a role in the binding of the lactose synthase complex. The mature protein consists of 123 amino acid residues (14 kD), and it has a three-dimensional structure with 1.7 Α° resolution, demonstrating four α-helices and a triple stranded antiparallel β-sheet. |
| General description: | α-Lactalbumin is a small, globular, whey protein that has been found in all milk studied to date. It is a metalloprotein of approximately 14 kDa produced in the mammary glands. |
| Preparation Note: | 0.9-1.4 mg/mL after reconstitution with 1 mL of water |
| RIDADR | NONH for all modes of transport |
| WGK Germany | 3 |
| Storage Temp. | −20°C |
| UNSPSC | 12352202 |