Lysostaphin from Staphylococcus staphylolyticus
SIGMA/L4402 - BioUltra, ≥97% (SDS-PAGE), Protein 40-60 % by biuret, ≥2,000 units/mg protein
Synonym: Glycyl-glycine Endopeptidase
CAS Number: 9011-93-2
MDL Number: MFCD00131556
Product Type: Chemical
Lysostaphin has endopeptidase activity, which cleaves polyglycine cross-links in the cellular wall of Staphylococcus species. Under certain conditions preparations may also exhibit hexosaminidase activity (specific for the glucosaminyl-muramic acid bond of the bacterial carbohydrate backbone, but does not react with S. aureus) and amidase activity.
Lysostaphin is supplied as a single polypeptide chain without the signal and propeptide regions.
SDS-PAGE (4–20%) of Lysostaphin (Cat. No. L4402) under reducing conditions and stained with Coomassie blue.
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: L4402-.5MG
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: L4402-2MG
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: L4402-5MG
| antibiotic activity spectrum | Gram-positive bacteria |
| application(s) | diagnostic assay manufacturing |
| assay | ≥97% (SDS-PAGE) |
| biological source | bacterial ( |
| composition | Protein, 40-60% biuret |
| concentration | 40—60% protein |
| form | lyophilized powder |
| mode of action | cell wall synthesis | interferes |
| mol wt | 25 kDa |
| product line | BioUltra |
| Quality Level | 200  |
| 300 |
|
| specific activity | ≥2,000 units/mg protein |
| storage temp. | −20°C |
| suitability | suitable for cell lysis |
| technique(s) | cell based assay: suitable |
| Biochem/physiol Actions: | Lysostaphin is a zinc endopeptidase with a molecular weight of approximately 25 kDa. Because lysostaphin cleaves the polyglycine cross-links in the peptidoglycan layer of the cell wall of Staphylococcus species it has been found useful for cell lysis and also as a potential anti-microbial therapeutic. pH Optimum for activity: ~7.5 |
| General description: | Chemical structure: peptide |
| General description: | Package size based on protein content |
| Other Notes: | One unit will reduce the turbidity (A620) of a suspension of Staphylococcus aureus cells from 0.250 to 0.125 in 10 min at pH 7.5 at 37 °C in a 6.0 ml reaction mixture. |
| Packaging: | 0.5, 2, 5 mg in glass bottle |
| Physical form: | Contains potassium phosphate buffer salts and sodium chloride |
| Preparation Note: | Affinity purified |
| Symbol |  GHS08 |
| Signal word | Danger |
| Hazard statements | H334 |
| Precautionary statements | P261 - P284 - P304 + P340 + P312 - P501 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥97% (SDS-PAGE) |
| activity | specific activity: ≥2,000 units/mg protein |
| Storage Temp. | −20°C |
| Enzyme Commission (EC) Number | 3.4.24.75 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |