Lipopolysaccharides from Escherichia coli O127:B8
SIGMA/L4516 - BioXtra, suitable for cell culture, γ-irradiated
Synonym: LPS
CAS Number: 93572-42-0
EC Number: 297-473-0
MDL Number: MFCD00212998
Product Type: Chemical
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| biological source | Escherichia coli (O127:B8) |
| form | lyophilized powder |
| impurities | <1% Protein (Lowry) |
| product line | BioXtra |
| purified by | gel-filtration chromatography |
| Quality Level | 200  |
| solubility | H2O: 5 mg/mL, slightly hazy |
| sterility | γ-irradiated |
| storage temp. | 2-8°C |
| technique(s) | cell culture | mammalian: suitable |
| Application: | Lipopolysaccharides (LPSs) are characteristic components of the cell wall of Gram-negative bacteria. LPS and its lipid A moiety stimulate cells of the innate immune system by the Toll-like receptor 4 (TLR4), a member of the Toll-like receptor protein family, which recognizes common pathogen-associated molecular-patterns (PAMPs). |
| Application: | Lipopolysaccharides from Escherichia coli 0127:B8 has been used: • to study the LPS-induced macrophage survival in mice • to elicit the secretion of cytokines by cattle PBMC • to study apoptosis of highly susceptible macrophage cells in a LPS-induced proinflammatory environment • to perform autophagy assay in BV2 and BV2-LC3 cells • for stimulation of human monocytes, macrophages and mouse microglia cells • to induce pentraxin-3 expression in 293T cells |
| Biochem/physiol Actions: | Lipopolysaccharides (LPSs) are characteristic components of the cell wall of Gram-negative bacteria. LPS and its lipid A moiety stimulate cells of the innate immune system by the Toll-like receptor 4 (TLR4). Being an immunogenic antigen, it enhances immune responses to soluble antigens. |
| Other Notes: | To gain a comprehensive understanding of our extensive range of Lipopolysaccharides for your research, we encourage you to visit our Carbohydrates Category page. |
| Preparation Note: | Lipopolysaccharides are supplied as lyophilized, γ-irradiated powders. To reconstitute, add 1 ml sterile balanced salt solution or tissue culture medium to the vial (1 mg) and gently swirl until the powder dissolves. Reconstituted product may be further diluted to desired working concentrations using sterile balanced salt solution or tissue culture medium. |
| Symbol |  GHS06 |
| Signal word | Danger |
| Hazard statements | H300 |
| Precautionary statements | P264 - P270 - P301 + P310 - P405 - P501 |
| Hazard Codes | Xn |
| Risk Statements | 20-21-22 |
| Safety Statements | 36 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352211 |