Anti-Lysyl Oxidase (C-terminal) antibody produced in rabbit
SIGMA/L4669 - ~1 mg/mL, affinity isolated antibody, buffered aqueous solution
Synonym: Anti-LOX; Anti-Protein lysine 6-oxidase
Product Type: Chemical
Enhanced Validation-By Recombinant Expression Antibody recognition: Anti-Lysyl Oxidase (C-terminal) antibody produced in Rabbit (Cat. No. L4669) is confirmed by western blot in the cell sample that has been altered to overexpress the target protein-HEK-293T over expressing Lysyl Oxidase (Lane 1) while no staining is detected in the wild type sample-HEK-293T (Lane 2). The antibody was developed with Goat Anti-Rabbit IgG, Peroxidase conjugate (Cat. No. A0545) and a chemiluminescent substrate. Antibody dilution: 1.5 μg/mL.
Immunohistochemistry Localization of lysyl oxidase (LOX) in human colon. Formalin-fixed paraffin-embedded tissue was stained with 20 μg/mL Anti-Lysyl Oxidase (C-terminal) (Cat. No. L4669) followed by Biotin-Goat Anti-Rabbit IgG/ExtrAvidin®-HRP (×20 magnification).
Immunohistochemistry Localization of LRRK2 in human kidney. Formalin-fixed paraffin-embedded tissue was stained with 30 μg/mL Anti-LRRK2 (C-terminal region) (Cat. No. L9918) followed by Biotin-Goat Anti-Rabbit IgG/ExtrAvidin®-HRP (×40 magnification).
Immunofluorescence Cytoplasmic localization of lysyl oxidase (LOX) in human fibroblast HS68 cells. Fixed cells were stained with 10 μg/mL Anti-Lysyl Oxidase (Cat. No. L4794) followed by Goat Anti-Rabbit IgG, Cy3™ conjugate (×40 magnification). Nuclear staining with DAPI.
Immunofluorescence Cytoplasmic localization of lysyl oxidase (LOX) in human fibroblast HS68 cells. Fixed cells were stained with 10 μg/mL Anti-Lysyl Oxidase (C-terminal) (Cat. No. L4669) followed by Goat Anti-Rabbit IgG, Cy3™ conjugate (×40 magnification). Nuclear staining with DAPI.
Immunoblotting Cell lysates of HEK-293T overexpressing human Lysyl Oxidase (LOX) (Lanes 1-3) and HEK293-T non-transfected (Lane 4), were separated on SDS-PAGE, blotted with Anti-Lysyl Oxidase (C-terminal) (Cat. No. L4669) and developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and chemiluminescent substrate. Lanes 1. Antibody: 1.5 μg/mL 2. Antibody: 0.75 μg/mL 3. Antibody: 1.5 μg/mL+ LOX immunizing peptide (human, 299-318) 4. Antibody: 1.5 μg/mL
Immunoblotting Cell lysates of human fibroblasts HS68 were separated on SDS-PAGE, blotted with 1.5 μg/mL Anti-Lysyl Oxidase (C-terminal) (Cat. No. L4669) and developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and chemiluminescent substrate.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1 mg/mL |
| conjugate | unconjugated |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation  |
| form | buffered aqueous solution |
| mol wt | antigen ~50 kDa |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | western blot: 1.5-3.0 mg/mL using HEK-293T cellls expressing human LOX |
| UniProt accession no. | P28300  |
| Application: | Anti-Lysyl Oxidase (C-terminal) antibody produced in rabbit has been used in: • western blotting • immunofluorescence staining • immunohistochemistry |
| Biochem/physiol Actions: | Altered LOX gene expression and activity are linked to skin aging and senescence. Decreased LOX gene expression and activity have been associated with severe connective tissue disorders such as Ehler-Danlos syndrome, cutis laxa, and Menkes′ syndrome. Increased LOX gene expression are associated with the development of fibrotic diseases that involve connective tissue remodeling, such as atherosclerosis, scleroderma and liver cirrhosis. LOX protein also plays an important role in breast cancer metastasis. |
| Biochem/physiol Actions: | Anti-Lysyl Oxidase (C-terminal) specifically recognizes human lysyl oxidase (LOX). |
| Biochem/physiol Actions: | LOX is implicated in various intracellular functions such as the regulation of cellular differentiation, cell migration and gene transcription. |
| Biochem/physiol Actions: | The gene LOX (lysyl oxidase) encodes an extracellular matrix remodeling enzyme that catalyzes the oxidation of primary amino group of peptidyl lysine to reactive peptidyl aldehydes. It is mainly involved in the oxidation of lysine residues in elastin and collagen, resulting in the formation of covalent cross-linkages, which stabilize these fibrous proteins. It also plays a role in several cellular processes such as developmental regulation, tumor suppression, cell motility, and cellular senescence. It is involved in hypoxia-induced metastasis and serves as a therapeutic target for the treatment of metastases. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | The gene LOX (lysyl oxidase) encodes a copper-dependent amine oxidase that belongs to LOX family of proteins. These proteins contain highly conserved C- terminal mature catalytic domains that include the copper binding site, the lysyl tyrosyl quinine (LTQ) cofactor residues, and the cytokine receptor like (CRL) domain. It is present in both intercellular and intracellular locations. |
| General description: | The LOX gene is located on the human chromosome at 5q23.1. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| Preparation Note: | For continuous use, store at 2–8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours. |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |