Anti-Lysyl Oxidase antibody produced in rabbit
SIGMA/L4794 - ~1 mg/mL, affinity isolated antibody, buffered aqueous solution
Synonym: Lysyl Oxidase Antibody; Lysyl Oxidase Antibody - Anti-Lysyl Oxidase antibody produced in rabbit; Anti-LOX; Anti-Protein lysine 6-oxidase
Product Type: Chemical
Enhanced Validation-By Recombinant Expression Antibody recognition: Anti-Lysyl Oxidase antibody produced in Rabbit (Cat. No. L4794) is confirmed by western blot in the cell sample that has been altered to overexpress the target protein-HEK-293T over expressing Lysyl Oxidase (Lane 1) while no staining is detected in the wild type sample-HEK-293T (Lane 2). The antibody was developed with Goat Anti-Rabbit IgG, Peroxidase conjugate (Cat. No. A0545) and a chemiluminescent substrate. Antibody dilution: 3 μg/mL.
Immunoblotting Cell lysates of HEK-293T overexpressing human Lysyl Oxidase (LOX) (Lanes 1-3) and HEK293-T non-transfected (Lane 4), were separated on SDS-PAGE, blotted with Anti-Lysyl Oxidase (Cat. No. L4794) and developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and chemiluminescent substrate. Lanes 1. Antibody: 3 μg/mL 2. Antibody: 1.5 μg/mL 3. Antibody: 3 μg/mL+ LOX immunizing peptide (human, 187-202) 4. Antibody: 3 μg/mL
Immunoblotting Cell lysates of human fibroblasts HS68 were separated on SDS-PAGE, blotted with 1.5 μg/mL Anti-Lysyl Oxidase (Cat. No. L4794) and developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and chemiluminescent substrate.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1 mg/mL |
| conjugate | unconjugated |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation  |
| form | buffered aqueous solution |
| mol wt | antigen ~50 kDa |
| Quality Level | 200 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | western blot: 1.5-3.0 μg/mL using HEK-293T cells expressing human LOX |
| UniProt accession no. | P28300  |
| Application: | Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below. Western Blotting (1 paper) |
| Biochem/physiol Actions: | Rabbit polyclonal anti-Lysyl Oxidase antibody specifically recognizes human lysyl oxidase (LOX). Detection of the LOX band by immunoblotting is specifically inhibited by the LOX immunizing peptide. |
| Biochem/physiol Actions: | The gene LOX (lysyl oxidase) encodes an extracellular matrix remodeling enzyme that catalyzes the oxidation of primary amino group of peptidyl lysine to reactive peptidyl aldehydes. It is mainly involved in the oxidation of lysine residues in elastin and collagen, resulting in the formation of covalent cross-linkages, which stabilize these fibrous proteins. It also plays a role in several cellular processes such as developmental regulation, tumor suppression, cell motility, and cellular senescence. It is involved in hypoxia-induced metastasis and serves as a therapeutic target for the treatment of metastases. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | The gene LOX (lysyl oxidase) encodes a copper-dependent amine oxidase that belongs to LOX family of proteins. These proteins contain highly conserved C- terminal mature catalytic domains that include the copper binding site, the lysyl tyrosyl quinine (LTQ) cofactor residues, and the cytokine receptor like (CRL) domain. It is present in both intercellular and intracellular locations. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |