Lectin from Triticum vulgaris (wheat)
SIGMA/L4895 - FITC conjugate, lyophilized powder
Synonym: WGA; Wheat germ agglutinin
Product Type: Chemical
Agglutination
assay: Lectin from Triticum vulgaris (wheat) (Cat. No.
L4895) at a concentration of ~1mg solid/mL was serially diluted (2-fold, top
to bottom of plate) and incubated with human erythrocytes type: A (4% v/v
RBC) to calculate Minimum agglutination titer (MAT). Agglutination is defined
as the inability of cells to form a tight button. PBS was used as blank.
assay: Lectin from Triticum vulgaris (wheat) (Cat. No.
L4895) at a concentration of ~1mg solid/mL was serially diluted (2-fold, top
to bottom of plate) and incubated with human erythrocytes type: A (4% v/v
RBC) to calculate Minimum agglutination titer (MAT). Agglutination is defined
as the inability of cells to form a tight button. PBS was used as blank.
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: L4895-10MG
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: L4895-2MG
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: L4895-5MG
| composition | Protein, ~50% Lowry |
| conjugate | FITC conjugate |
| extent of labeling | ~2 mol FITC per mol protein |
| form | lyophilized powder |
| potency | <50 μg/mL agglutination activity |
| Quality Level | 200  |
| storage temp. | 2-8°C |
| Application: | For the fluorescent detection of glycoproteins containing β(1→4)-N-acetyl- |
| Application: | Lectin from Triticum vulgaris (wheat) has been used: • to treat transfected cells for immunofluorescence • to stereotaxically inject into mice for neuronal tracing • in immunohistochemistry for the analysis of myocytes |
| Biochem/physiol Actions: | Lectin plays a key role in glycoconjugate characterizing, imaging and targeting. Its use in a microarray assay, enable efficient glycome profiling. In plants and fungi, lectin defends against pathogens/feeders. Lectin also plays a major role in host recognition and tissue adhesion. |
| Biochem/physiol Actions: | WGA is not blood group specific but has an affinity for N-acetyl-β- |
| General description: | Lectins are carbohydrate-binding proteins. They are omnipresent and usually found in fungi, plants and animals. The secondary structure of this protein is rich in β-strands and possesses carbohydrate binding sites on the surface. |
| Packaging: | 2, 5, 10 mg in glass bottle |
| Packaging: | Package size based on protein content. |
| Physical form: | Contains phosphate buffer salts |
| Preparation Note: | Conjugates are prepared from affinity purified lectin. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352202 |