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L-Leucine Dehydrogenase from Bacillus cereus

SIGMA/L5135 - lyophilized powder, ≥60 units/mg protein

Synonym: L-Leucine:NAD+ oxidoreductase (deaminating)

CAS Number: 9082-71-7
MDL Number: MFCD00147461
Product Type: Chemical
Catalog Number PKG Qty. Price Quantity
45-L5135-25UN 25 units
 $1060.00
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This image is provided for informative purposes only and does not represent an actual product label. It should not be used as a substitute for official product labeling.

 

form lyophilized powder
mol wt 245 kDa
Quality Level 200 
specific activity ≥60 units/mg protein
storage temp. −20°C
Application: L-Leucine Dehydrogenase from Bacillus cereus has been used to determine the branched-chain amino acids (BCAA) spectrophotometrically in serum samples.
Biochem/physiol Actions: Leucine Dehydrogenase is a nicotinamide adenine dinucleotide hydrogen (NADH)-dependent oxidoreductase. It is involved in catalyzing the reductive amination of aliphatic 2-oxo-acids to their respective L-amino acids.
General description: L-Leucine Dehydrogenase is a member of the amino acid dehydrogenase family.
Other Notes: contains lysine
Other Notes: One unit will convert 1.0 μmole of L‑leucine to α-ketoisocaproate per min at pH 10.5 at 37 °C.
RIDADR NONH for all modes of transport
WGK Germany WGK 3
Flash Point(F) Not applicable
Flash Point(C) Not applicable
activity specific activity: ≥60 units/mg protein
Storage Temp. −20°C
Enzyme Commission (EC) Number 1.4.1.9   ( BRENDA  | IUBMB  )
UNSPSC 12352204

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