Lectin from Triticum vulgaris (wheat)
SIGMA/L5142 - biotin conjugate, lyophilized powder
Synonym: WGA; Wheat germ agglutinin
Product Type: Chemical
Agglutination assay: Lectin from Triticum vulgaris (wheat) (Cat. No. L5142) at a concentration of ~1mg solid/mL was serially diluted (2-fold, top to bottom of plate) and incubated with human erythrocytes type: A (4% v/v RBC) to calculate Minimum agglutination titer (MAT). Agglutination is defined as the inability of cells to form a tight button. PBS was used as blank.
| composition | Protein, ≥80% E |
| conjugate | biotin conjugate |
| extent of labeling | 2-4 mol biotin per mol protein |
| form | lyophilized powder |
| Quality Level | 200  |
| storage temp. | 2-8°C |
| Application: | Lectin from Triticum vulgaris (wheat) has been used: • in lectin binding assay of samples from intrauterine stages of filarial parasites • in the histological studies of mice myocytes • for the generation bead-biotin-WGA complex with Dynabeads® M-280 Streptavidin magnetic beads for lectin magnetic separation of Toxoplasma gondii oocytes from water samples |
| Biochem/physiol Actions: | WGA is not blood group specific but has an affinity for N-acetyl-β- |
| General description: | Wheat germ lectin (WGA) from Triticum vulgaris exists as a homodimer and has multiple N-acetyl glucosamine (GlcNAc) binding sites. It is resistant to protein denaturation agents and is used for the detection of O-GlcNAc proteins. WGA binding to N-glycolylneuraminic acid (Neu5Ac) may modulate pro-inflammatory immune response. |
| Legal Information: | Dynabeads is a registered trademark of Invitrogen Dynal AS Corp |
| Packaging: | 1, 5 mg in serum bottle |
| Packaging: | Package size based on protein content. |
| Physical form: | Contains sodium citrate |
| Preparation Note: | Conjugates are prepared from affinity purified lectin. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352202 |