α-Lactalbumin from bovine milk
SIGMA/L6385 - For use as a marker in SDS-PAGE
Synonym: alpha-lactalbumin
CAS Number: 9051-29-0
MDL Number: MFCD00162025
Product Type: Chemical
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| biological source | bovine milk |
| concentration | >5 mg per vial protein (biuret) |
| form | powder |
| mol wt | ~14.2 kDa |
| packaging | vial of 5 mg |
| Quality Level | 200  |
| solubility | H2O: soluble 10 mg/mL |
| storage temp. | 2-8°C |
| technique(s) | electrophoresis: suitable |
| UniProt accession no. | P00711  |
| Application: | α-Lactalbumin from bovine milk is suitable for use: • as an electrophoresis marker, with a molar mass of approximately 14,200Da • in a study to investigate selective binding of proteins on charged surface iron oxide nanoparticles via reverse charge parity model α-Lactalbumin was used in the reduction of the antigenicity of whey proteins by lactic acid fermentation. |
| Biochem/physiol Actions: | Alters the substrate specificity of galactosyltransferase to increase the rate of lactose formation; the complex of galactosyltransferase and α-lactalbumin is called lactose synthase. |
| Biochem/physiol Actions: | Alters the substrate specificity of galactosyltransferase to increase the rate of lactose formation; the complex of galactosyltransferase and α-lactalbumin is called lactose synthase. Site-directed mutagenesis of Asp87 or Asp88 to Ala completely abolishes the strong calcium binding affinity and reduces the stimulation of lactose synthase to <3.5% of the maximal rate. |
| Biochem/physiol Actions: | α-Lactalbumin consists of a single polypeptide chain with 8 cysteines which form disulfide bridges. α-Lactalbumin binds several metal ions, including calcium, which is thought to play a role in the regeneration of native α-lactalbumin from the reduced, denatured form. α-Lactalbumin also has a distinct zinc binding site that is thought to play a role in the binding of the lactose synthase complex. The mature protein consists of 123 amino acid residues (14 kD), and it has a three-dimensional structure with 1.7 Angstrom resolution, demonstrating four a-helices and a triple stranded antiparallel β-sheet. |
| General description: | α-Lactalbumin is a small, globular, whey protein that has been found in all milk studied to date. It is a metalloprotein of approximately 14 kDa produced in the mammary glands. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | 3 |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352202 |