Concanavalin A from Canavalia ensiformis (Jack bean)

SIGMA/L6397 - peroxidase conjugate, lyophilized powder

Synonym: ConA

Product Type: Chemical

Catalog Number	PKG Qty.	Price


45-L6397-1MG	1 mg	$294.00 1/EA	Add To Favorites
45-L6397-5MG	5 mg	$1280.00 1/EA	Add To Favorites

Lanes 1. Urokinase from human urine (Sigma U0633) (glcNAc,Fuc) 2. Factor IX Human (Sigma F0806) (galNAc, Glc, glcNAc, Fuc) 3. Complement component C1q from human serum (Sigma C1740) (galNAc, Gal) 4. a1-Antitrypsin from human plasma (Sigma A3106) (glcNac) 5. Fetuin (Sigma F2379) (glcNac) 6. Invertase glycoprotein Standard (Sigma I0408) (glcNac) 7. IgG1 Monoclonal Anti-Flag M2 Ab produced in mouse (Sigma F3165) (Gal, Man, glcNac, Fuc) 8. IgG2a Monoclonal Anti-AMY1A Ab produced in mouse (Sigma WH0000276M4) (Gal, Man, glcNac, Fuc) 9. Recombinant Human t-Plasminogen Activator/tPA (R&D Systems 7449-SE-010) (glcNAc, Fuc) 10. Epidermal growth factor receptor human (Sigma E3641) (glcNAc) 11. Apolipoprotein C-III from human plasma (Sigma A3106) 12. Thrombospondin from human platelets (Calbiochem 605225)

Properties
Descriptions
Safety Info.
Basic Data

biological source	Canavalia ensiformis (jack bean)
conjugate	peroxidase conjugate
form	lyophilized powder
impurities	carbohydrate, essentially free
Quality Level	200
storage temp.	−20°C
technique(s)	blood typing: suitable
	fractionation: suitable

Analysis Note:	Where reported, agglutination activity is expressed in μg/ml and is determined from serial dilutions in phosphate buffered saline, pH 6.8, containing Ca²⁺ and Mn²⁺ of a 1 mg per mL solution. This activity is the lowest concentration to agglutinate a 2% suspension of human erythrocytes after 1 hr incubation at 25 °C.
Application:	Detects glycoproteins containing α-D-mannose, α-D-glucose when used with appropriate peroxidase substrate
Application:	General Western Blot Protocol: • Glycoprotein sample size: 500ng • Lectin Concentration: 0.1ug/ml Load samples at 500 ng of glycoprotein per lane • Run 4-20% Bis-Tris SDS page gel • Transfer gel to a PVDF membrane • Block membrane for 1 hr at RT with RIPA buffer (R0278 Sigma) • Incubate HRP lectin at 0.1ug/ml with RIPA buffer for 2 hours at RT • Wash membrane 5 x 5 minutes with 25ml RIPA buffer • Detect using chemiluminescent substrate (CPS1-120)
Biochem/physiol Actions:	Con A is not blood group specific but has an affinity for terminal α-D-mannosyl and α-D-glucosyl residues. Ca²⁺ and Mn²⁺ ions are required for activity. Con A dissociates into dimers at pH 5.6 or below. Between pH 5.8 and pH 7.0, Con A exists as a tetramer; above pH 7.0 higher aggregates are formed. Con A exhibits mitogenic activity which is dependent on its degree of aggregation. Succinylation results in an active dimeric form which remains a dimer above pH 5.6.
Other Notes:	One unit will form 1 mg purpurogallin in 20 sec from pyrogallol at pH 6.0 at 20 °C.
Packaging:	Package size based on protein content
Physical form:	Lyophilized powder containing tris-citrate buffer salts and trace calcium and manganese
Preparation Note:	Prepared from peroxidase type VI (P8375), using the method of Wilson and Nakane, which promotes conjugation but prevents the interaction between Con A and peroxidase sugar residues.

Symbol	GHS08
Signal word	Danger
Hazard statements	H317 - H334 - H361
Precautionary statements	P201 - P202 - P280 - P302 + P352 - P304 + P340 + P312 - P308 + P313
Hazard Codes	Xn
Risk Statements	42/43
Safety Statements	24/25-36
RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point(F)	Not applicable
Flash Point(C)	Not applicable

Storage Temp.	−20°C
UNSPSC	12352202

To Order From a Quote