Monoclonal Anti-Human Lambda Light Chains (Bound and Free) antibody produced in mouse
SIGMA/L6522 - clone HP-6054, ascites fluid
Synonym: Monoclonal Anti-Human Lambda Light Chains (Bound and Free)
MDL Number: MFCD00162516
Product Type: Chemical
ELISA Monoclonal Anti-Human λ Light Chains (Bound and Free) antibody produced in Mouse, Clone: HP-6054 (Cat. No. L6522), followed by 1:500 Anti-Mouse IgG (Fab specific)-Peroxidase antibody produced in Goat (Cat. No. A9917), was serially diluted to detect Human λ Light Chains by indirect ELISA. Reactivity is observed with B.J λ, Human IgG and Human IgG1,λ but not with Human IgG1k and B.J k.
| antibody form | ascites fluid |
| antibody product type | secondary antibodies |
| biological source | mouse |
| clone | HP-6054, monoclonal |
| conjugate | unconjugated |
| contains | 15 mM sodium azide |
| isotype | IgG2a |
| Quality Level | 200  |
| shipped in | dry ice |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry: suitable |
| immunoprecipitation (IP): suitable | |
| indirect ELISA: 1:1,000 using using 2 μg/ml of human IgG myeloma containing lambda light chains as the coating protein | |
| radioimmunoassay: suitable | |
| western blot: suitable |
| Application: | Monoclonal Anti-Human Lambda Light Chains (Bound and Free) antibody produced in mouse has been used: • in immunohistochemistry • in enzyme-linked immunosorbent assay (ELISA) assay • in immunohistology • in radioimmunoassay (RIA) • in precipitation assays • in immunoblotting • as capture antibodies in quantitative ELISA |
| Biochem/physiol Actions: | Immunoglobulins (Ig) are produced by the B cells and play crucial role in host humoral immune response. A typical immunoglobulin consists of two heavy chains and two light chains. The two light chains are either κ or the λ chain. The B cells express one type of light chain during their life time. |
| Biochem/physiol Actions: | The antibody is specific for human lambda (λ) light chains. This antibody is reactive with intact IgG (λ), IgA (λ), free lambda light chains and Bence-Jones lambda light chains. It is non-reactive with kappa light chains. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti-Human Lambda Light Chains (mouse IgG2a isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Immunoglobulins are composed of two heavy and two light polypeptide chains held together by noncovalent forces and usually by interchain disulfide bridges. The various types of human (and other mammalian) immunoglobulins contain one of the two existing light chain types, either κ or λ in which multiple structural differences are reflected in antigenic variety, mainly the N-terminal (variable) domain of the chains. |
| Immunogen: | Purified human IgG myeloma proteins covalently coupled to polyaminostyrene (PAS) microbeads. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |