Lectin from Dolichos biflorus (horse gram)
SIGMA/L6533 - biotin conjugate, buffered aqueous solution
Synonym: Dolichos biflorus agglutinin; DBA
Product Type: Chemical
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| composition | Protein, ≥2.5 mg/mL biuret |
| conjugate | biotin conjugate |
| extent of labeling | ≥3 mol biotin per mol lectin |
| form | buffered aqueous solution |
| Quality Level | 200  |
| storage temp. | −20°C |
| Application: | Lectin from Dolichos biflorus (horse gram) has been used: • to characterize mouse uterine natural killer (uNK) cells • in double staining with lectins to localize nuclear factor-κB (NF-κB) activation • as a species-specific gonocyte marker for the determination of donor cell colonization • to label undifferentiated spermatogonia/cat testicular tissue |
| Biochem/physiol Actions: | DBA has anti-A1 human blood group specificity, useful for distinguishing between A1 and A2 blood types. DBA has strong anti-Cad activity, and has an affinity for terminal N-acetyl-α- |
| Biochem/physiol Actions: | Dolichos biflorus agglutinin (DBA) possesses binding activity for which it is used as a marker binucleate cells and can indicate the state of their differentiation. It serves as a label for primordial germ cells (PGCs), gonocytes, pre-spermatogonia and spermatogonia. |
| General description: | Lectin from Dolichos biflorus (horse gram) is a heterotetrameric glycoprotein with a molecular mass of around 113 kDa. Dolichos biflorus agglutinin (DBA) is specific for terminal α-linked N-acetyl-D-galactosamine (GalNAc). It is a dietary lectin. |
| Packaging: | Sold on the basis of mg active conjugate. |
| Physical form: | contains 10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide, 0.1 mM Ca2+ |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352202 |