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Lectin from Dolichos biflorus (horse gram)

SIGMA/L6533 - biotin conjugate, buffered aqueous solution

Synonym: Dolichos biflorus agglutinin; DBA

Product Type: Chemical
Catalog Number PKG Qty. Price Quantity
45-L6533-5MG 5 mg
 $520.00
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This image is provided for informative purposes only and does not represent an actual product label. It should not be used as a substitute for official product labeling.

 

composition Protein, ≥2.5 mg/mL biuret
conjugate biotin conjugate
extent of labeling ≥3 mol biotin per mol lectin
form buffered aqueous solution
Quality Level 200 
storage temp. −20°C
Application: Lectin from Dolichos biflorus (horse gram) has been used:
• to characterize mouse uterine natural killer (uNK) cells
• in double staining with lectins to localize nuclear factor-κB (NF-κB) activation
• as a species-specific gonocyte marker for the determination of donor cell colonization
• to label undifferentiated spermatogonia/cat testicular tissue
Biochem/physiol Actions: DBA has anti-A1 human blood group specificity, useful for distinguishing between A1 and A2 blood types. DBA has strong anti-Cad activity, and has an affinity for terminal N-acetyl-α-D-galactosaminyl residues. Conjugates are prepared from affinity purified lectin.
Biochem/physiol Actions: Dolichos biflorus agglutinin (DBA) possesses binding activity for which it is used as a marker binucleate cells and can indicate the state of their differentiation. It serves as a label for primordial germ cells (PGCs), gonocytes, pre-spermatogonia and spermatogonia.
General description: Lectin from Dolichos biflorus (horse gram) is a heterotetrameric glycoprotein with a molecular mass of around 113 kDa. Dolichos biflorus agglutinin (DBA) is specific for terminal α-linked N-acetyl-D-galactosamine (GalNAc). It is a dietary lectin.
Packaging: Sold on the basis of mg active conjugate.
Physical form: contains 10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide, 0.1 mM Ca2+
RIDADR NONH for all modes of transport
WGK Germany WGK 3
Flash Point(F) Not applicable
Flash Point(C) Not applicable
Storage Temp. −20°C
UNSPSC 12352202

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