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Lipoxidase from Glycine max (soybean)

SIGMA/L6632 - Type V, ammonium sulfate suspension, 500,000-1,000,000 units/mg protein

Synonym: Linoleate:oxygen oxidoreductase; Lipoxygenase

CAS Number: 9029-60-1
EC Number: 232-853-1
MDL Number: MFCD00131527
Product Type: Chemical
Catalog Number PKG Qty. Price Quantity
45-L6632-1MU 1000000 units
 $113.00
1/EA		 Add To Favorites
45-L6632-5MU 5000000 units
 $396.00
1/EA		 Add To Favorites
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form ammonium sulfate suspension
mol wt 94 kDa
Quality Level 200 
specific activity 500,000-1,000,000 units/mg protein
storage temp. 2-8°C
type Type V
Analysis Note: Protein determined by biuret.
Application: Lipoxidase, or lipoxygenase, from Glycine max (soybean) has been used for the modification of low density lipoprotein, isolated from human plasma.
Application: The soybean enzyme will use arachidonic acid as a substrate, with ~ 15% of the activity indicated using linoleic acid as the substrate; the product of arachidonic acid oxidation is 12- or 15-hydroperoxyarachidonic acid (12-HPETE or 15-HPETE).
Biochem/physiol Actions: Catalyzes the hydroperoxidation of lipids containing a cis,cis-1,4-pentadiene structure.
Other Notes: One unit will cause an increase in A234 of 0.001 per min at pH 9.0 at 25 °C when linoleic acid is the substrate in 3.0 ml volume (1 cm light path). One A234 unit is equivalent to the oxidation of 0.12 μmole of linoleic acid.
Physical form: Suspension in 2.3 M (NH4)2SO4 solution, pH approx. 6.0
Preparation Note: Prepared by ion exchange chromatography and hydrophobic interaction chromatography.
Symbol GHS08  GHS08
Signal word Danger
Hazard statements H334
Precautionary statements P261 - P284 - P501
RIDADR NONH for all modes of transport
WGK Germany WGK 1
Flash Point(F) Not applicable
Flash Point(C) Not applicable
activity specific activity: 500,000-1,000,000 units/mg protein
Storage Temp. 2-8°C
Enzyme Commission (EC) Number 1.13.11.12   ( BRENDA  | IUBMB  )
UNSPSC 12352204

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