LuminoCt® qPCR ReadyMix™
SIGMA/L6669 - For fast probe-based quantitative PCR
Synonym: LuminoCt® qPCR ReadyMix™; LuminoCt®
Product Type: Chemical
Representative packaging
Standard Curve. The standard curve was generated on an ABI 7500 Fast instrument by plotting the threshold cycle versus the log of the initial quantity of DNA for each reaction.
Amplification Plot. Log-fold dilutions of human fibroblast genomic DNA (108 to 103 fg) were assayed in triplicate using LuminoCt qPCR ReadyMix and a dual-labeled fluorescent probe and primers targeting the 18S ribosomal RNA gene. Assays were performed on an ABI 7500 Fast qPCR instrument according to the manufacturers recommended protocol.
| application(s) | agriculture |
| color | colorless |
| compatibility | Bio-Rad iCycler iQ |
| Bio-Rad iQ 5 | |
| Bio-Rad MyiQ ( ) | |
| for use with ABI 5700 | |
| for use with ABI 7000 | |
| for use with ABI 7300 | |
| for use with ABI 7500 | |
| for use with ABI 7500 Fast | |
| for use with ABI 7700 | |
| for use with ABI 7900 | |
| for use with ABI 7900 Fast | |
| for use with ABI 7900 HT | |
| for use with ABI StepOne | |
| for use with ABI StepOnePlus | |
| for use with ABI ViiA 7 | |
| for use with Bio-Rad CFX384 | |
| for use with Bio-Rad CFX96 | |
| for use with Bio-Rad MiniOpticon | |
| for use with Bio-Rad MJ Chromo4 | |
| for use with Bio-Rad MJ Opticon | |
| for use with Bio-Rad MJ Opticon 2 | |
| for use with Bio-Rad MJ Opticon Cepheid SmartCycler | |
| for use with Eppendorf® Mastercycler ep realplex | |
| for use with Eppendorf® Mastercycler ep realplex2 s | |
| for use with Illumina Eco qPCR | |
| for use with Qiagen Corbett Rotor-Gene 3000 | |
| for use with Qiagen Corbett Rotor-Gene 6000 | |
| for use with Qiagen Corbett Rotor-Gene Q | |
| for use with Roche LightCycler 480 | |
| for use with Strategene Mx3000P | |
| for use with Strategene Mx3005P | |
| for use with Strategene Mx4000 | |
| detection method | probe-based |
| feature | dNTPs included |
| hotstart | |
| form | liquid |
| input | purified DNA |
| Quality Level | 200  |
| shipped in | wet ice |
| storage temp. | −20°C |
| suitability | suitable for (quantitative PCR) |
| technique(s) | qPCR: suitable |
| usage | sufficient for 100 reactions |
| sufficient for 2000 reactions | |
| sufficient for 500 reactions |
| Application: | LuminoCt® qPCR ReadyMix™ has been used in 2-step quantitative reverse transcription polymerase chain reaction (qRT-PCR) assay to quantify the reverse transcription product or cDNA for gene expression analysis. It has also been used in qPCR for quantifying the RNA extracted from rhinoviruses. |
| Features and Benefits: | • Assay results in as little as 25 minutes • Designed to deliver unsurpassed accuracy, precision, and sensitivity • Extensive design and validation of ReadyMix chemistry virtually eliminates the need to optimize assay parameters |
| Features and Benefits: | • Ideal for rapid, high-throughput 2-step qPCR protocols • The master mix allows consistency and reproducibility from one reaction to the next • Reduced preparation time and the risk of contamination from multiple pipetting steps • Compatible with commonly available real-time instrument platforms for probe-based qPCR assays • Require small amplicons (<200 bp) for optimal results • Compatible with commercial primer sets, including TaqMan® assays • JumpStart™ Taq DNA polymerase allows room temperature set-up and the hot-start mechanism prevents primer-dimer and non-specific product formation |
| General description: | Inclusion of JumpStart™ Taq antibody in the ReadyMix™ eliminates polymerase activity at ambient temperatures without causing the performance issues associated with chemically modified hot-start Taq. This allows for very rapid activation of the Taq and delivers unparalleled assay sensitivity, while allowing for benchtop reaction setup. • Kit is designed to perform probe-based qPCR assays on commonly available real-time instrument platforms. • ReadyMix requires the addition of reference dye (provided in kit) when being used on real-time instruments that require ROX passive reference dye for normalization of qPCR assays. • Kit is not compatible with qPCR instruments that utilize glass capillary tubes. |
| General description: | LuminoCt® qPCR ReadyMix combines the performance enhancements of our JumpStart™ Taq antibody for hot start PCR with the convenience of an easy-to-use reaction mixture. |
| Legal Information: | Eppendorf is a registered trademark of Eppendorf AG |
| Legal Information: | JumpStart is a trademark of Sigma-Aldrich Co. LLC |
| Legal Information: | LuminoCt is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Legal Information: | ReadyMix is a trademark of Sigma-Aldrich Co. LLC |
| Legal Information: | TaqMan is a registered trademark of Roche Molecular Systems, Inc. |
| Other Notes: | • Commercially available 5′ nuclease assays (e.g., TaqMan® Assays) are compatible with LuminoCt ReadyMix. • At a minimum, use software to design primers and probes for assays and ensure that qPCR amplicons are <200 bp. • Sigma recommends designing primers and probes with Beacon Designer software To order primers and probes from Sigma, visit www.sigma.com/oligos  |
| Packaging: | LuminoCt qPCR ReadyMix, Catalog Number L5794, contains optimized concentrations of Tris-HCl, pH 8.3, KCl, dNTPs (dATP, dCTP, dGTP, TTP), stabilizers, MgCl2 and JumpStart Taq DNA Polymerase. Provided as 100, 500 and 2000 reactions (25 μL mix in a 50 μL reaction volume). 100x ROX internal reference dye, Catalog Number R4526. Optional, for use with machines compatible with an internal reference dye, e.g., ABI and Stratagene. Default reaction volume is 50 μL 100RXN is packaged as 1 X 2.5 mL 500RXN is packaged as 1 X 12.5 mL 2000RXN is packaged as 1 X 50 mL |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Storage Temp. | −20°C |
| UNSPSC | 41106300 |
| Components | 100X ROX internal reference dye |