Lactacystin
SIGMA/L6785 - ≥90% (HPLC)
CAS Number: 133343-34-7
Empirical Formula (Hill Notation): C15H24N2O7S
Molecular Weight: 376.43
MDL Number: MFCD01076525
Linear Formula: C15H24N2O7S
Product Type: Chemical
| assay | ≥90% (HPLC) |
| form | powder |
| InChI | 1S/C15H24N2O7S/c1-6(2)10( |
| InChI key | DAQAKHDKYAWHCG-RWTHQLGUSA |
| potency | 4 nM Ki (proteasome inhibitor) |
| Quality Level | 200  |
| SMILES string | CC(C)[C@H](O)[C@]1(NC(=O) |
| solubility | water: 10 mg/mL, clear, colorless |
| storage temp. | −20°C |
| Application: | Lactacystin has been used: • as a proteasome inhibitor to inhibit protein degradation • to inhibit proteasomal activity of cells for live cell imaging • to block proteasomal proteolysis in human monocyte-derived dendritic cells (MoDCs) for 24 h • to provide unilateral injection to animals to induce nigrostriatal lesions |
| Biochem/physiol Actions: | Lactacystin can block the development of cell cycle and stimulate differentiation in a murine neuroblastoma cell line. It can serve as a precursor for clasto-lactacystin β-lactone. Cell-permeable and irreversible proteasome inhibitor (Ki = 4nM). Inhibits NF-kB activation (IC50 = 10mM). Induces neurite outgrowth in neuro2A mouse neuroblastoma cells. |
| General description: | Lactacystin is an antibiotic and a metabolite of Streptomyces spp. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥90% (HPLC) |
| Storage Temp. | −20°C |
| UNSPSC | 12352200 |