Monoclonal Anti-LSD1 (AOF2) antibody produced in mouse
SIGMA/L7293 - ~2 mg/mL, clone LSD1-12, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-Amine oxidase, flavin-containing, 2; Anti-BHC110; Anti-BRAF-HDAC complex protein 110; Anti-KIAA0601 protein; Anti-Lysine-specific demethylase 1
MDL Number: MFCD09039194
Product Type: Chemical
Western Blotting Total cell extract of MCF7 cells was separated on SDS-PAGE and blotted with Monoclonal Anti-LSD1 (Cat. No. L7293). The antibody was developed with Goat Anti-Mouse IgG, Peroxidase conjugate (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1. 1 μg/mL antibody 2. 0.5 μg/mL antibody
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | LSD1-12, monoclonal |
| concentration | ~2 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG1 |
| mol wt | antigen ~110 kDa |
| Quality Level | 300  |
| shipped in | dry ice |
| species reactivity | mouse, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | western blot: 0.5-1 μg/mL using MCF7 total cell extract |
| Application: | Monoclonal Anti-LSD1 (AOF2) antibody produced in mouse has been used: • immunofluorescence • immunoblotting • immunoprecipitation • immunocytochemistry |
| Biochem/physiol Actions: | Lysine Specific Demethylase 1 (LSD1) is a component of several histone deacetylase complexes that function through modifying chromatin structure to repress transcription.LSD1 action can signal either activation or repression of transcription. Demethylation of histone H3 Lys4 by LSD1 leads to transcriptional repression of target genes, while demethylation of histone H3 at Lys9 leads to depression of androgen receptor target genes. The activity of LSD1 (AOF2) is regulated by its associated factors namely, corepressor to the REST (RE1 silencing transcription factor/neural restrictive silencing factor)(CoREST). This, in turn, promotes demethylation by enhancing the association of LSD1 with the nucleosomes and also protects LSD1 from proteasomal degradation. LSD1 is a component of nucleosome remodeling and deacetylase (NuRD). It regulates neuronal development and favors neural stem cell proliferation. It highly expressed in acute myeloid leukemia and many solid tumors. |
| Biochem/physiol Actions: | Monoclonal Anti-LSD1 (AOF2) recognizes human and mouse LSD1. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti-LSD1 (AOF2) (mouse IgG1 isotype) is derived from the hybridoma LSD1-12 produced by the fusion of mouse myeloma cells (NS1) and splenocytes from BALB/c mice. Lysine specific demethylase 1 (LSD1) also known as amine oxidase flavin-containing domain 2 (AOF2) () is a lysine-specific demethylase. It is a nuclear protein containing a SWIRM (Swi3p, Rsc8p and Moira) domain, a flavin adenine dinucleotide (FAD)-binding motif and an amine oxidase domain. The LSD1 gene is mapped to human chromosome 1p36.12. LSD1 is referred to by additional names including KDM1A protein, BHC110(BRAF-HDAC complex protein 110) and nuclear polyamine oxidase (NPAO)(). |
| Immunogen: | synthetic peptide corresponding to amino acids 832-847 of human LSD1/BHC110. This sequence is identical in bovine, rat, mouse, and frog. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| Preparation Note: | For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |