Lectin from Arachis hypogaea (peanut)
SIGMA/L7381 - FITC conjugate, lyophilized powder
Synonym: PNA; Peanut agglutinin
Product Type: Chemical
Agglutination
assay: Lectin from Arachis hypogaea (peanut) (Cat. No.
L7381) at a concentration of ~1mg solid/mL was serially diluted (2-fold, top
to bottom of plate) and incubated with human erythrocytes type: O (4% v/v
RBC) to calculate Minimum agglutination titer (MAT). Agglutination is defined
as the inability of cells to form a tight button. PBS was used as blank.
assay: Lectin from Arachis hypogaea (peanut) (Cat. No.
L7381) at a concentration of ~1mg solid/mL was serially diluted (2-fold, top
to bottom of plate) and incubated with human erythrocytes type: O (4% v/v
RBC) to calculate Minimum agglutination titer (MAT). Agglutination is defined
as the inability of cells to form a tight button. PBS was used as blank.
| composition | Protein, ~10% Lowry |
| conjugate | FITC conjugate |
| extent of labeling | 4-8 mol FITC per mol protein |
| form | lyophilized powder |
| Quality Level | 200  |
| storage temp. | −20°C |
| Application: | Lectin from Arachis hypogaea (peanut) has been used: • to determine the acrosomal status (presence or absence of acrosomal matrix corresponding to intact or acrosome-reacted spermatozoa) on viable sperm cells • to probe cryosections of mediastinal lymph nodes (mLNs) with fluorochrome-labeled PNA and anti-B220 to detect germinal centers (GCs) • to visualize the cone outer segments of pre-treated retinal sections |
| Biochem/physiol Actions: | Lectin is known to be useful in glycoconjugate characterizing, imaging and targeting. Its use in a microarray assay, enable efficient glycome profiling. This is because of its specific interaction with oligosaccharides, glycoproteins and glycolipids. In plants and fungi, lectin defends against pathogens/feeders. Lectin participates in host recognition and tissue adhesion, thereby aids in the pathogenesis of microorganism. |
| Biochem/physiol Actions: | PNA does not agglutinate normal human erythrocytes, but strongly agglutinates neuraminidase treated erythrocytes. PNA has potent anti-T activity similar to the anti-T antibody in human sera. The lectin can be used to distinguish between human lymphocyte subsets. |
| General description: | Lectins are carbohydrate-binding proteins, omnipresent, found in fungi, plants and animals. The structure of lectin is diversely studied in plants and animals. The secondary structure of this protein is rich in β-strands and possesses a carbohydrate binding sites on the surface. |
| Packaging: | 1, 2, 5 mg in glass bottle |
| Physical form: | Contains phosphate buffer salts and NaCl |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352202 |