Anti-LIS1 antibody, Mouse monoclonal
SIGMA/L7391 - clone LIS1-338, purified from hybridoma cell culture
MDL Number: MFCD03457563
Product Type: Chemical
Western Blotting Whole extract of Rat Barin cells were separated on SDS-PAGE and probed with Anti-LIS1 antibody, Mouse monoclonal, Clone: LIS1-338 (Cat. No. L7391). The antibody was developed using 1:10,000 Anti-Mouse IgG (Fab specific)-Peroxidase antibody produced in Goat (Cat. No. A9917). Lanes 1. 0.5 μg/mL antibody 2. 1 μg/mL antibody 3. Negative Control
| antibody form | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | LIS1-338, monoclonal |
| concentration | ~2 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| InChI | 1S/C21H39O7P/c1-2-3-4-5-6 |
| InChI key | ZQTAMPRZFOOEEP-AVQMFFATSA |
| isotype | IgG1 |
| mol wt | antigen 46 kDa |
| shipped in | dry ice |
| SMILES string | CCCCCC=CCC=CCCCCCCCC( |
| species reactivity | zebrafish, chicken, rat, human, bovine, mouse |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoprecipitation (IP): suitable |
| microarray: suitable | |
| western blot: 0.5-1 μg/mL using a rat brain cytosol preparation | |
| UniProt accession no. | P43034   |
| Application: | Monoclonal Anti-LIS1 antibody produced in mouse has been used in protein pulldown assay and immunofluorescence. |
| Biochem/physiol Actions: | By immunoblotting, the antibody prefers the phosphorylated form of the LIS1 molecule (46 kDa). |
| Biochem/physiol Actions: | LIS1 is involved in cell regulation, including the β subunits of G-proteins (Gβ). This protein motif is likely to mediate protein-protein interactions. LIS1 is a protein that is highly conserved during evolution; bovine, mouse, and chicken proteins exceed 99% similarity. |
| Biochem/physiol Actions: | The heterotrimeric enzyme, platelet-activating factor acteylhydrolase (PAFAH), catalyzes the cleavage of acetyl group at the SN-2 position of platelet-activating factor. The α subunit encoded by the gene PAFAH1B1 (platelet activating factor acetylhydrolase 1b regulatory subunit 1) interacts with tubulin and affects microtubule dynamics. Mutations in this gene have been linked to Miller–Dieker lissencephaly, a human brain malformation characterized by a smooth cerebral surface and a disordered organization of the cortical layers resulting from a defect in neuronal migration. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti-LIS1 (mouse IgG1 isotype) is derived from the LIS1-338 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c mouse immunized with a recombinant human LIS1 protein. The LIS1 protein (46 kDa) contains seven WD (tryptophan-aspartic acid) repeats, a motif shared by at least 140 known proteins. |
| General description: | The gene PAFAH1B1 (platelet activating factor acetylhydrolase 1b regulatory subunit 1) encodes a protein named LIS1 (lissencephaly-1) that forms the α subunit of the intracellular Ib isoform of platelet-activating factor acteylhydrolase (PAFAH). The gene is mapped to human chromosome 17p13.3. |
| Immunogen: | recombinant human LIS1 protein. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| Hazard Codes | Xn |
| Risk Statements | 21/22 |
| Safety Statements | 36/37-60 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |