Lipoxidase from Glycine max (soybean)
SIGMA/L7395 - Type I-B, lyophilized powder, ≥50,000 units/mg solid
Synonym: 15-lipoxygenase; LOX A; Linoleate:oxygen oxidoreductase; Lipoxygenase
CAS Number: 9029-60-1
EC Number: 232-853-1
MDL Number: MFCD00131527
Product Type: Chemical
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: L7395-150MU
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: L7395-15MU
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: L7395-75MU
| form | lyophilized powder |
| mol wt | 94 kDa |
| Quality Level | 200  |
| shipped in | wet ice |
| specific activity | ≥50,000 units/mg solid |
| storage temp. | −20°C |
| type | Type I-B |
| Application: | Lipoxidase from Glycine max (soybean) has been used: • to study the effects of 2-(α- • as a representative enzyme for lipoxygenases in microplate assay platform to screen for enzymes degrading wood extractives • as a positive control in lipoxygenase inhibitory activity assay |
| Application: | The soybean enzyme will use arachidonic acid as a substrate, with ~ 15% of the activity indicated using linoleic acid as the substrate; the product of arachidonic acid oxidation is 12- or 15-hydroperoxyarachidonic acid (12-HPETE or 15-HPETE). |
| Biochem/physiol Actions: | Catalyzes the hydroperoxidation of lipids containing a cis,cis-1,4-pentadiene structure. |
| Biochem/physiol Actions: | Lipoxidases are deactivated by heat treatment near 100°C for 5 to 10 minutes. Studies show that gamma irradiation treatment decreases the specific activity of soybean lipoxygenases. These enzymes are used in studies related to asthma and allergies. Lipoxidases are applicable for industrial bio-transformations such as in the production of aroma fine chemicals. |
| General description: | Lipoxidases (LOXs), or lipoxygenases, are nonheme iron proteins. Lipoxygenases are widely present in plants, some microorganisms, and animals. They are categorized as LOX-1, LOX-2, and LOX-3 with respect to pH and optimum temperature, substrate specificity, and level of inhibition by different agents. |
| Other Notes: | One unit will cause an increase in A234 of 0.001 per min at pH 9.0 at 25 °C when linoleic acid is the substrate in 3.0 ml volume (1 cm light path). One A234 unit is equivalent to the oxidation of 0.12 μmole of linoleic acid. |
| Packaging: | 15000000, 75000000, 150000000 units in poly bottle |
| Physical form: | Contains stabilizer and NaCl |
| Symbol |  GHS08 |
| Signal word | Danger |
| Hazard statements | H334 |
| Precautionary statements | P261 - P284 - P501 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: ≥50,000 units/mg solid |
| Storage Temp. | −20°C |
| Enzyme Commission (EC) Number | 1.13.11.12 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |