LB Broth (Lennox)
SIGMA/L7658 - EZMix powder microbial growth medium
Synonym: Lennox broth
Product Type: Chemical
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This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: L7658-1KG
| application(s) | agriculture food and beverages microbiology |
| description | quick-dissolve, dust-free formulation |
| form | powder |
| grade | Molecular Biology |
| pH | 6.8-7.2(2.5% solution) |
| Quality Level | 200  |
| sterility | non-sterile |
| storage temp. | room temp |
| suitability | nonselective for Escherichia coli |
| nonselective for coliforms | |
| technique(s) | microbiological culture: suitable |
| Application: | Suitable for non-selective cultivation of E. coli strains for cloning, DNA plasmid production and production of recombinant proteins. Also suitable for selective cultivation when appropriate antibiotics are added, including those that require low-salt conditions, such as Zeocin®. |
| Features and Benefits: | Lennox LB EZMix powder provides: • Granulated, dust-free format for safer handling and faster mixing • Convenient small package to eliminate weighing • Easy scale-up using larger package sizes • Standard formulation |
| General description: | Lennox LB is a highly-referenced microbial growth medium used for the cultivation of E. coli. This nutrient-rich microbial broth contains peptides, amino acids, water-soluble vitamins, and carbohydrates in a low-salt formulation. |
| Legal Information: | Zeocin is a registered trademark of Cayla Sarl |
| Other Notes: | *Note some of the product sizes available are given in liquid measure. This is a powder format product and those sizes reflect the final reconstituted volume. 10g/L Tryptone 5 g/L Yeast Extract 5 g/L NaCl 0.6 g/L inert binder (EZMix formulation only) |
| Packaging: | 1 kg in poly bottle |
| Preparation Note: | 1. Suspend 20.6 g in 1 L of distilled water. 2. Autoclave for 15 minutes at 121 °C. To prepare Lennox L Broth: Add 1 g glucose and proceed with preparation instructions as above. To prepare the medium of Enquist and Sternberg: Aseptically add 10 ml of sterile 1M magnesium sulfate after autoclaving. |
| Preparation Note: | Stir to suspend 20.6g powder in 1L water. Autoclave for 15 minutes at 121°C to sterilize. Allow to cool before making additions, such as antibiotics (if desired). |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | room temp |
| UNSPSC | 41106200 |