Lectin from Ulex europaeus (gorse, furze)
SIGMA/L8146 - peroxidase conjugate, lyophilized powder
Synonym: Ulex europaeus agglutinin; UEA-I
Product Type: Chemical
Lanes 1. Urokinase from human urine (Sigma U0633) 2. Factor IX Human (Sigma F0806) 3. Complement component C1q from human serum (Sigma C1740) (galNAc, Gal) 4. a1-Antitrypsin from human plasma (Sigma A3106)
5. Fetuin (Sigma F2379) 6. Invertase glycoprotein Standard (Sigma I0408) 7. IgG1 Monoclonal Anti-Flag M2 Ab produced in mouse (Sigma F3165) 8. IgG2a Monoclonal Anti-AMY1A Ab produced in mouse (Sigma WH0000276M4) 9. Recombinant Human t-Plasminogen Activator/tPA (R&D Systems 7449-SE-010) 10. Epidermal growth factor receptor human (Sigma E3641) 11. Apolipoprotein C-III from human plasma (Sigma A3106) 12. Thrombospondin from human platelets (Calbiochem 605225)
5. Fetuin (Sigma F2379) 6. Invertase glycoprotein Standard (Sigma I0408) 7. IgG1 Monoclonal Anti-Flag M2 Ab produced in mouse (Sigma F3165) 8. IgG2a Monoclonal Anti-AMY1A Ab produced in mouse (Sigma WH0000276M4) 9. Recombinant Human t-Plasminogen Activator/tPA (R&D Systems 7449-SE-010) 10. Epidermal growth factor receptor human (Sigma E3641) 11. Apolipoprotein C-III from human plasma (Sigma A3106) 12. Thrombospondin from human platelets (Calbiochem 605225)
Agglutination
assay: Lectin from Ulex europaeus (gorse, furze) (Cat.
No. L8146) at a concentration of ~1mg solid/mL was serially diluted (2-fold,
top to bottom of plate) and incubated with human erythrocytes type: A (4% v/v
RBC) to calculate Minimum agglutination titer (MAT). Agglutination is defined
as the inability of cells to form a tight button. PBS was used as blank.
assay: Lectin from Ulex europaeus (gorse, furze) (Cat.
No. L8146) at a concentration of ~1mg solid/mL was serially diluted (2-fold,
top to bottom of plate) and incubated with human erythrocytes type: A (4% v/v
RBC) to calculate Minimum agglutination titer (MAT). Agglutination is defined
as the inability of cells to form a tight button. PBS was used as blank.
| assay | 70.00-90.00% (Lowry) |
| biological source | Ulex europaeus |
| composition | Protein, ~80% Lowry |
| conjugate | peroxidase conjugate |
| form | lyophilized powder |
| peroxidase activity | ≥15 units/mg protein |
| potency | <40 μg/mL agglutination activity |
| Quality Level | 200  |
| storage temp. | 2-8°C |
| technique(s) | western blot: suitable |
| Analysis Note: | Agglutination activity is expressed in μg/ml and is determined from serial dilutions in phosphate buffered saline, pH 6.8, of a 1 mg/ml solution. This activity is the lowest concentration to agglutinate a 2% suspension of human blood group O erythrocytes after 1 hour incubation at 25 °C. |
| Application: | General Western Blot Protocol: • Glycoprotein sample size: 500ng • Lectin Concentration: 0.1ug/ml
|
| Biochem/physiol Actions: | Ulex europaeus agglutinin has anti-H blood group specificity. There are two types of lectin: UEA I has an affinity for |
| Other Notes: | Conjugates are prepared from affinity purified lectin. |
| Other Notes: | One unit will form 1.0 mg purpurogallin in 20 sec from pyrogallol at pH 6.0 at 20 °C. |
| Packaging: | 0.5, 1 mg in serum bottle |
| Packaging: | Package size based on protein content. |
| Physical form: | Contains sodium citrate buffer salts |
| Preparation Note: | Prepared from peroxidase, Type VI (P8375), using the two-step glutaraldehyde method of Avrameas and Ternyck. Repurified by affinity chromatography after conjugation. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | 70.00-90.00% (Lowry) |
| activity | peroxidase activity: ≥15 units/mg protein |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352202 |