Luciferase from Vibrio fischeri (Photobacterium f)
SIGMA/L8507 - lyophilized powder
Synonym: Bacterial Luciferase; Luciferase from Photobacterium fischeri
CAS Number: 9014-00-0
EC Number: 232-751-7
MDL Number: MFCD00131545
Product Type: Chemical
ATP is hydrolyzed and light is emitted when firefly luciferase catalyzes the oxidation of D-luciferin. Reaction (1) is reversible and the equilibrium lies far to the right. Reaction (2) is essentially irreversible. When ATP is the limiting reagent, the light emitted is proportional to the ATP present.
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: L8507-100MG
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: L8507-500MG
| composition | Protein, ~40% biuret |
| form | lyophilized powder |
| Quality Level | 200  |
| storage temp. | −20°C |
| Application: | Luciferase from Vibrio fischeri has been used in a study to assess kinetics of light emission and oxygen consumption by bioluminescent bacteria. It has also been used in a study to investigate the sensitivity of dark mutants of various strains of luminescent bacteria to reactive oxygen species. |
| Features and Benefits: | Partially purified, soluble extracts containing FMN-dependent luciferase and NADH- and NADPH-dependent FMN reductases. Produces light in a system containing FMN, NADH or NADPH, and n-decyl aldehyde. |
| Other Notes: | ATCC No. 7744 balance primarily buffer salts and stabilizer. |
| Packaging: | 100, 500 mg in poly bottle |
| Physical form: | Partially purified lyophilized powder |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| Enzyme Commission (EC) Number | 1.14.14.3 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |