Lectin from Phaseolus vulgaris (red kidney bean)
SIGMA/L8754 - Phytohemagglutinin PHA-P, lyophilized powder
Synonym: Phaseolus vulgaris agglutinin; PHA
EC Number: 232-718-7
Product Type: Chemical
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: L8754-25MG
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: L8754-50MG
| biological source | Phaseolus vulgaris |
| form | lyophilized powder |
| impurities | salt, free |
| potency | ≤10 μg/mL mitogenic activity |
| ≤62.5 μg per mL agglutination activity | |
| Quality Level | 300  |
| storage temp. | 2-8°C |
| Analysis Note: | Agglutination activity is expressed in mg/ml and is determined from serial dilutions of a 1 mg/ml solution. This activity is the lowest concentration to agglutinate a suspension of 2% human erythrocytes in phosphate buffered saline, pH 6.8, after 1 hr incubation at 25 °C. Mitogenic activity is determined by BrdU incorporation in HeLa cell cultures. |
| Application: | It has also been used as an antigen to measure in vivo T-cell-mediated immune responsiveness. |
| Application: | Lectin from Phaseolus vulgaris (red kidney bean) has been used in phytohemagglutinin (PHA) assay and during assessment of cellular and humoral immunity. |
| Biochem/physiol Actions: | PHA consists of two molecular species, an erythroagglutinin (PHA-E) which has low mitogenic activity and high erythroagglutinin activity, and leucoagglutinin (PHA-L) which has high mitogenic and leucoagglutinating activity, but very low erythroagglutinating activity. PHA-E is not blood group specific, but agglutination can be inhibited by certain oligosaccharides. PHA-P is the protein form of PHA prior to separation and purification of erythroagglutinin and leucoagglutinin. PHA-M is the mucoprotein form. Conjugates are prepared from the corresponding purified lectins. |
| General description: | PHAs are carbohydrate-binding proteins. There are several types of PHAs identified, predominantly in leguminous plants. |
| General description: | Phytohaemagglutinin (PHA, or phytohemagglutinin) is a lectin found in plants (esp. in legumes). It is obtained from Phaseolus vulgaris (red kidney beans) and acts as a mitogen in human leucocyte culture. |
| Packaging: | 25, 50 mg in poly bottle |
| Packaging: | 5 mg in glass bottle |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352202 |