Lectin from Phytolacca americana (pokeweed)

SIGMA/L8777 - lyophilized powder, BioReagent, suitable for cell culture

Synonym: PWM; Pokeweed mitogen

MDL Number: MFCD00161973
Product Type: Chemical

Catalog Number	PKG Qty.	Price	Quantity


45-L8777-5MG	5 mg	$128.00 1/EA		Add To Favorites
45-L8777-10MG	10 mg	$241.00 1/EA		Add To Favorites

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Properties
Descriptions
Safety Info.
Basic Data

form	lyophilized powder
potency	≤0.3 μg/mL mitogenic activity
product line	BioReagent
Quality Level	200
solubility	PBS: 1 mg/mL
sterility	aseptically filled
storage temp.	2-8°C
technique(s)	cell culture \| mammalian: suitable

Analysis Note:	Mitogenic activity was determined by BrdU incorporation in HeLa cell cultures.
Analysis Note:	Partially purified TCA precipitate. Essentially salt-free.
Application:	Lectin from Phytolacca americana (pokeweed) is used for proliferation Assay. (Cellular proliferative responses were measured by the incorporation of 3H thymidine in newly synthesized DNA, using a conventional assay).
Biochem/physiol Actions:	Lectins are carbohydrate-binding proteins and are highly specific for sugar moieties. Pokeweed lectin (PL) is a lectin specific for N-acetylglucosamine-containing saccharides that stimulate peripheral lymphocytes to undergo mitosis by binding to their cell surfaces. Four types of lectins are isolated from the roots of pokeweed (Phytolacca americana). They contain homologous domains but have different molecular sizes and biological properties.
Biochem/physiol Actions:	PWM is extracted from pokeweed roots. This protein has hemagglutinating, leukoagglutinating, and mitogenic properties. PWM is not blood group specific, but has affinity for N-acetyl-β-D-glucosamine oligomers.
General description:	Lectins are proteins of plant origin, which have the ability to bind to certain neuronal surface glycoproteins and glycolipids. This ability of theirs helps them to be used as neuroanatomical tracers, as they are taken and transported in a retrograde or anterograde fashion.
Packaging:	5, 10 mg in glass bottle

Symbol	GHS08
Signal word	Danger
Hazard statements	H317 - H334
Precautionary statements	P261 - P280 - P342 + P311
Hazard Codes	Xn
Risk Statements	42/43
Safety Statements	36
RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point(F)	Not applicable
Flash Point(C)	Not applicable

Storage Temp.	2-8°C
UNSPSC	12352202

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