Lectin from Phaseolus vulgaris (red kidney bean)
SIGMA/L9017 - BioXtra, Phytohemagglutinin PHA-P, purified by affinity chromatography, lyophilized powder
Synonym: Phaseolus vulgaris agglutinin; PHA
EC Number: 232-718-7
Product Type: Chemical
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| biological source | Phaseolus vulgaris |
| form | lyophilized powder |
| impurities | salt, essentially free |
| potency | ≤17 μg per mL agglutination activity |
| product line | BioXtra |
| purified by | affinity chromatography |
| Quality Level | 300  |
| storage temp. | 2-8°C |
| Analysis Note: | Agglutination activity is expressed in mg/mL and is determined from serial dilutions of a 1 mg/mL solution. This activity is the lowest concentration to agglutinate a suspension of 2% human erythrocytes in phosphate buffered saline, pH 6.8 after 1 hr incubation at 25 °C. Mitogenic activity is determined by BrdU incorporation. |
| Analysis Note: | Mitogenic activity at ≤10 μg per mL. |
| Application: | Lectin from Phaseolus vulgaris (red kidney bean) has been used: • for phytohemagglutinin (PHA) swelling assay • for lymphocyte activation assay • to evaluate cell-mediated immune response |
| Biochem/physiol Actions: | Lectin agglutinates cells and precipitates polysaccharides and glycoproteins. It has anti-cancer, anti-human immunodeficiency virus (anti-HIV) and anti-microbial infection properties. Lectin is associated with toxic allergens and hemagglutinins. Lectin inhibits mucosal atrophy, lowers type 2 diabetes and obesity, enhances nutrient absorption and regulates drug targeting. |
| Biochem/physiol Actions: | PHA consists of two molecular species, an erythroagglutinin (PHA-E) which has low mitogenic activity and high erythroagglutinin activity, and leucoagglutinin (PHA-L) which has high mitogenic and leucoagglutinating activity, but very low erythroagglutinating activity. PHA-E is not blood group specific, but agglutination can be inhibited by certain oligosaccharides. PHA-P is the protein form of PHA prior to separation and purification of erythroagglutinin and leucoagglutinin. PHA-M is the mucoprotein form. Conjugates are prepared from the corresponding purified lectins. |
| General description: | Lectins are carbohydrate-binding proteins, that contains four subunits with a molecular weight of 125 kDa. Leucoagglutinin PHA-L is a plant lectin, which is transported by the neurons in the central nervous system. |
| Packaging: | 1, 5, 10 mg in glass bottle |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352202 |