Lysostaphin from Staphylococcus simulans
SIGMA/L9043 - recombinant, expressed in E. coli, lyophilized powder
Synonym: Glycyl-glycine Endopeptidase
CAS Number: 9011-93-2
MDL Number: MFCD00131556
Product Type: Chemical
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: L9043-5MG
| application(s) | life science and biopharma |
| assay | ≥90% (HPLC) |
| biological source | bacterial |
| form | lyophilized powder |
| Quality Level | 200  |
| recombinant | expressed in E. coli |
| shipped in | dry ice |
| specific activity | ≥3000 unit/mg solid |
| storage temp. | −20°C |
| suitability | suitable for sequence analysis test |
| technique(s) | molecular cloning: suitable |
| Application: | Lysostaphin from Staphylococcus simulans has been used in a study to assess molecular cloning, sequencing, and expression of lytM, a unique autolytic gene of Staphylococcus aureus. It has also been used in a study to investigate the sequence analysis of a Staphylococcus aureus gene encoding a peptidoglycan hydrolase activity. |
| Biochem/physiol Actions: | A protein complex with highly specific lytic activity against Staphylococcus species, including Staphylococcus aureus. |
| Biochem/physiol Actions: | Lysostaphin possesses a lytic action against Staphylococcus aureus, which is used to treat antibiotic-resistant staphylococcal infections. It has the properties of enzymes, such as glycylglycine endopeptidase, endo-β-N-acetyl glucosamidase and N-acetyl muramyl- |
| Biochem/physiol Actions: | The antimicrobial activity of lysostaphin, a metalloendopeptidase expressed by Staphylococcus simulans, is enhanced by binding to the C′ terminus of the membrane-associated Trap protein of Staphylococcus aureus. |
| General description: | Lysostaphin, a zinc metalloenzyme, is mainly secreted as a proenzyme. It acts as an anti-staphylococcal agent and is highly stable at pH 4 and 5°C. |
| Packaging: | 5 mg in glass bottle |
| Symbol |  GHS08 |
| Signal word | Danger |
| Hazard statements | H334 |
| Precautionary statements | P261 - P284 - P501 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥90% (HPLC) |
| activity | specific activity: ≥3000 unit/mg solid |
| Storage Temp. | −20°C |
| Enzyme Commission (EC) Number | 3.4.24.75 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |