Anti-Laminin antibody produced in rabbit
SIGMA/L9393 - 0.5 mg/mL, affinity isolated antibody, buffered aqueous solution
Synonym: Laminin Antibody
MDL Number: MFCD00162586
Product Type: Chemical
Immunohistochemistry-immunofluorescence JOURNAL CITATION: Meningeal cells and glia establish a permissive environment for axon regeneration after spinal cord injury in newts. By: Zukor, K. A., Kent, D. T., et al. in Neural Dev, 2011. PubMed ID: 21205291 Image collected and cropped by CiteAb from the following publication, (http://neuraldevelopment.biomedcentral.com/articles/10.1186/1749-8104-6-1), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Immunohistochemistry-immunofluorescence JOURNAL CITATION: Yap- and Cdc42-dependent nephrogenesis and morphogenesis during mouse kidney development. By: Reginensi, A., Scott, R. P., et al. in PLoS Genet, 2013. PubMed ID: 23555292 Image collected and cropped by CiteAb from the following publication, (http://dx.plos.org/10.1371/journal.pgen.1003380), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Immunohistochemistry JOURNAL CITATION: Notch pathway modulation on bone marrow-derived vascular precursor cells regulates their angiogenic and wound healing potential. By: Caiado, F., Real, C., et al. in PLoS One, 2008. PubMed ID: 19015735 Image collected and cropped by CiteAb from the following publication, (http://dx.plos.org/10.1371/journal.pone.0003752), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Enhanced Validation-By Independent Antibodies Formalin-fixed, paraffin-embedded Human Tongue sections stained with 1:30 Rabbit Anti-Laminin (Cat. No. L9393) (A). The antibody was developed using Goat Anti-Rabbit IgG, FITC conjugate (Cat. No. F9887), and 40 μg/mL Anti-Laminin antibody, Mouse monoclonal, Clone: LAM-89 (Cat. No. SAB4200719) (B). The antibody was developed using Goat Anti-Mouse IgG, Cy3™ conjugate. Results Two Anti-Laminin antibodies L9393 (A) and SAB4200719 (B) target different regions of Laminin show similar staining profiles between the two antibodies, demonstrating Independent Antibody Verification.
Immunohistochemistry Staining of formalin-fixed, paraffin-embedded Human Tongue sections with 1:30 Rabbit Anti-Laminin (Cat. No. L9393). The antibody was developed using Goat Anti-Rabbit IgG, FITC conjugate (Cat. No. F9887).
Immunohistochemistry Staining of formalin-fixed, paraffin-embedded Human tonsil sections with 1:50 Rabbit Anti-Laminin (Cat. No. L9393). The antibody was developed using Goat Anti-Rabbit IgG, Cy3™ conjugate.
Immunoblotting Laminin from Engelbreth-Holm-Swarm murine sarcoma basement membrane (Cat. No. L2020) was separated on SDS-PAGE and probed with Rabbit Anti-Laminin (Cat. No. L9393). The antibody was developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate. Lanes 1. antibody dilution 1:1,000 2. antibody dilution 1:2,000 3. antibody dilution 1:4,000 4. antibody dilution 1:8,000 5. antibody dilution 1:16,000 6. antibody dilution 1:32,000 7. Negative control: without first antibody
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| application(s) | research pathology |
| biological source | rabbit |
| clone | polyclonal |
| concentration | 0.5 mg/mL |
| conjugate | unconjugated |
| enhanced validation | independent Learn more about Antibody Enhanced Validation  |
| form | buffered aqueous solution |
| Quality Level | 300 |
| shipped in | dry ice |
| species reactivity | animal, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | dot blot: 1:1,000 |
| immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:30 using human and animal tissues | |
| microarray: suitable | |
| UniProt accession no. | P25391  |
| Application: | Anti-Laminin antibody has been used in immunohistochemical staining and immunohistochemistry. |
| Application: | Rabbit Anti-Laminin antibody may be used in immunohistochemistry for marking blood vessel walls in different species, classification of various disease processes involving basement membranes, identification of the origin of human tumors and their classification, and for distinguishing between non-invasive and invasive lesions. The antibody may be used to monitor levels of laminin in biological fluids and for experimental production of basement membrane lesions in vivo. A working dilution of at least 1:1,000 was determined by a dot blot immunoassay using laminin at 50 ng per dot. A working dilution of at least 1:25 was determined by indirect immunohistology using formalin-fixed, paraffin-embedded human and animal tissues. |
| Biochem/physiol Actions: | LAMA1 (laminin subunit α 1) is involved in several diseases like cancer, infections, inflammatory diseases and autoimmune disorders. Mutations in LAMA1 result in cerebellar dysplasia and cysts with and without retinal dystrophy. LAMA1 is also essential in the assembly of basement membrane and early growth of embryo. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Laminin is a ubiquitous non-collagenous connective tissue glycoprotein that is a major constituent of basement membranes. Laminins are extracellular matrix proteins. The LAMA1 (laminin subunit α 1) gene is mapped to human chromosome 18p11. The antibody is isolated from antiserum by immunospecific methods of purification. Antigen specific isolation removes essentially all rabbit serum proteins, including immunoglobulins that do not specifically bind to laminin. |
| Immunogen: | Laminin isolated from the basement membrane of Englebreth Holm-Swarm (EHS) mouse sarcoma. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% BSA and 15 mM sodium azide. |
| Specificity: | Specificity of the anti-laminin antibodies is determined by indirect immunofluorescent labeling of formalin-fixed, paraffin-embedded human or animal tissue sections, and by dot blot immunoassay. By indirect immunofluorescence the antibody demonstrates specific basement membrane staining of enzymatically unmasked human and animal tissue. In the dot blot immunoassay the rabbit anti-laminin antibody reacts with laminin but not with fibronectin, vitronectin, collagen IV, or chondroitin sulfate types A, B, and C. The affinity isolated antibody to laminin will react with laminin of human, mammal, avian, reptilian, and amphibian sources. |
| Storage and Stability: | For continuous use, store at 2-8 °C for up to one month. For extended storage, the solution may be frozen in working aliquots. Repeated freezing and thawing, or storage in "frost-free" freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify by centrifugation before use. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |