Luciferase from Photinus pyralis (firefly)
SIGMA/L9420 - recombinant, expressed in E. coli, buffered aqueous solution, ≥10×1010 units/mg protein
Synonym: Luciferase firefly
CAS Number: 61970-00-1
MDL Number: MFCD00131546
Product Type: Chemical
ATP is hydrolyzed and light is emitted when firefly luciferase catalyzes the oxidation of D-luciferin. Reaction (1) is reversible and the equilibrium lies far to the right. Reaction (2) is essentially irreversible. When ATP is the limiting reagent, the light emitted is proportional to the ATP present.
| assay | ≥98% (SDS-PAGE) |
| form | buffered aqueous solution |
| mol wt | 62 kDa |
| Quality Level | 200  |
| recombinant | expressed in E. coli |
| shipped in | dry ice |
| specific activity | ≥10×1010 units/mg protein |
| storage temp. | −20°C |
| Application: | Luciferase from Photinus pyralis (firefly) has been used as a component of lysis solution to measure luminescence signals, as part of the study to determine chemical signals which can activate the karrikin insensitive 2 (KAI2) pathway. |
| General description: | Firefly luciferase catalyzes the reaction of luciferin with ATP and leads to the production of yellow-green light. The enzyme has a molecular weight of 62kDa and is expressed in peroxisomes. Luciferase is considered as a model to study protein–anesthetic interactions. Firefly luciferase is highly useful in cell biology and molecular biology, as a reporter of gene function and for the quantification of ATP. |
| Other Notes: | One luciferase enzyme unit will produce one Relative Light Unit (RLU) at 20-25 °C over a 10 second period, measured in 100 μl assay mixture containing 40 pmol ATP and 15 nmol luciferin in Tris-glycine buffer, pH 7.6, using a GloMax 20/20 Luminometer. Unit Definition Conversion Factor: There are approximately 9000 Relative Light Units (RLU) per one traditional Light Unit that uses a peak height equivalent to 0.02 μCi of 14C in a PPO/POPOP cocktail. |
| Physical form: | Supplied a a solution in 25mM Tris-acetate, pH 7.8 , 1mM EDTA, 1mM DTT, 0.2M ammonium sulfate, 15% glycerol and 30% ethylene glycol |
| Symbol |   GHS07,GHS08 |
| Signal word | Warning |
| Hazard statements | H302 - H373 |
| Precautionary statements | P260 - P264 - P270 - P301 + P312 - P314 - P501 |
| Hazard Codes | Xn |
| Risk Statements | 22 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | 231.8 °F |
| Flash Point(C) | 111 °C |
| Purity | ≥98% (SDS-PAGE) |
| activity | specific activity: ≥10×1010 units/mg protein |
| Storage Temp. | −20°C |
| Enzyme Commission (EC) Number | 1.13.12.7 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |