Lipase from Pseudomonas sp.
SIGMA/L9518 - Type XIII, lyophilized powder, ≥15 units/mg solid
Synonym: Extracellular lipase; Triacylglycerol ester hydrolase; lip; Triacylglycerol acylhydrolase; Triacylglycerol lipase
CAS Number: 9004-02-8
EC Number: 232-669-1
MDL Number: MFCD00131509
Product Type: Chemical
Figure 1. Stability (powder form); kept under dry conditions
Figure 2. Stability (liquid form); in 20 mK K-phophate buffer, pH 7.5 (containing 2.0 mM MgCl2, 0.5 mM EDTA. Na3, 0.005% NaN3)
Figure 3. pH Activity; 37 °C, in 0.1 M Britton-Robinson buffer
Figure 4. Temperature activity; in 0.1 M K-phosphate buffer, pH 7.0
Figure 5. pH Stability; 25 °C, 20-hr treatment with 0.1 M Britton-Robinson buffer
Figure 6. Thermal stability; 10-min. treatment with 0.1 M K-phosphate buffer, pH 7.0
| composition | Protein, 40-65% biuret |
| form | lyophilized powder |
| mol wt | ~134 kDa |
| Quality Level | 200  |
| specific activity | ≥15 units/mg solid |
| storage temp. | 2-8°C |
| type | Type XIII |
| Analysis Note: | Protein determined by biuret. |
| Application: | Lipase from Pseudomonas sp. has been used:• for enzymatic determination of triglyceride in serum when coupled with L-α- glycerophosphate oxidase (G3O-301, G3O-311, G3O-321) and glycerol kinase (GYK-301, GYK-311). • in a study to assess enzymatic synthesis of biodiesel from palm oil assisted by microwave irradiation. • in determining the triglyceride content for identifying the role of gut bacteria in host health. • to determine the effect of lipase on high-density lipoprotein 2 (HDL2) and plasma in vitro. |
| Biochem/physiol Actions: | Lipase from Pseudomonas was shown to inhibit monocyte chemotaxis in human peripheral blood. It functions by employing chymotrypsin-like hydrolysis, involving a histidine base, a serine nucleophile, and aspartic acid. Lipases are also crucial for lipid transport. Elevated serum lipase levels are often observed in pancreatitis. Lipases are hydrolytic enzymes which break down triacylglycerides into free fatty acids and glycerols. |
| Biochem/physiol Actions: | Tri-, di-, and monoglycerides are hydrolyzed (in decreasing order of rate). |
| General description: | Research area: Cell Signaling Lipases are part of the α/β-hydrolase fold superfamily of enzymes and are expressed in various tissues. For instance, hepatic lipases are found in the liver, lipoprotein lipase is located on the vascular endothelial surface, hormone-sensitive lipases are present in adipocytes, and pancreatic lipase is situated in the small intestine. |
| General description: | Isoelectric point : 5.95 -/+0.05 Inhibitors : Hg++, Ag+, ionic detergents Optimum pH : 7.0 - 9.0 Optimum temperature : 45 - 50oC pH Stability : pH 7.0 - 9.0 (25oC, 20hr) Thermal stability : below 55oC (pH 7.0, 10min) |
| Other Notes: | One unit will produce 1.0 μmole of glycerol from a triglyceride per min at pH 7.0 at 37 °C in the presence of bovine serum albumin. |
| Packaging: | 500, 1000 units in poly bottle |
| Physical form: | Lyophilized powder containing Mg+2, sodium cholate, and bovine serum albumin as stabilizers |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: ≥15 units/mg solid |
| Storage Temp. | 2-8°C |
| Enzyme Commission (EC) Number | 3.1.1.34 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |