Leukemia Inhibitory Factor human, animal component free
SIGMA/L9545 - recombinant, expressed in rice, 500 μg/mL
Synonym: Leukemia Inhibitory Factor human; LIF; rLIF
MDL Number: MFCD00163950
Product Type: Chemical
Doubling time of human neural stem cells cultured in animal-free recombinant human LIF. H9-embryonic-derived neural stem cells were maintained in DMEM/F12, 2 mM Glutamax, 25 ng/mL bFGF and 1% N2 on poly-L-ornithine/laminin-coated plates. Cells were plated at a starting density of 0.1–0.2 × 105 viable cells/cm2 in either N2 growth medium or N2 growth medium supplemented with rice-derived rhLIF (L9545) for 96 h in 48-well plates coated with the same attachment matrix. Doubling time of cells was calculated using the formula LN(2)/μ where μ is equal to LN (final cell density/initial cell density)/1/time (in hours). Addition of rice-derived rhLIF induced a significant increase in viable cell density after 96 h in H9-derived NSCs (P = 0.00006). Data presented is cumulative from 3 experiments, 8 replicates per experiment.
| assay | >95% (SDS-PAGE) |
| concentration | 500 μg/mL |
| impurities | <0.05 EU/μg endotoxin |
| potency | >1 x 108 units/mg ED<sub>50</sub> |
| Quality Level | 200  |
| recombinant | expressed in rice |
| shipped in | dry ice |
| storage temp. | −70°C |
| technique(s) | cell culture | mammalian: suitable |
| UniProt accession no. | P15018  |
| Biochem/physiol Actions: | Leukemia Inhibitory Factor (LIF) is a pleiotropic glycoprotein originally described to inhibit the proliferation of the murine myeloid leukemic cell line M1, while inducing differentiation into macrophages. Other activities were later identified to LIF, which is known by a variety of synonyms, including DIF, D-factor, DIA, DRF, CNDF, HILDA, HSF-III, and MLPLI. Human LIF exerts its actions through a receptor comprising a 190 kDa LIF-binding α-chain (130 kDa, mouse) and a 130 kDa signal-transducing β-chain (gp130), which is shared with CNTF, OSM, L-6 and IL-11. As such, it is a member of the gp130 family of the cytokine receptor superfamily. LIF receptors have been identified on several cells, including monocytes, liver, placenta and embryonic stem cells. Natural LIF is heavily glycosylated, showing an apparent molecular weight of 32 kDa to 62 kDa, depending on the source, but absence of glycosylation appears not to affect its biological activity. A single gene encodes LIF, which is secreted as a single chain glycoprotein containing 180 amino acids for human or mouse with a conserved disulfide bond. Human and mouse LIF share 78% sequence homology. Human LIF can activate mouse cells, but mouse LIF cannot activate human cells. |
| Biochem/physiol Actions: | Leukemia Inhibitory Factor (LIF) is a pleiotropic glycoprotein that inhibits the proliferation of the murine myeloid leukemic cell line M1, while inducing differentiation into macrophages. Human and mouse LIF share 78% sequence homology. Human LIF can activate mouse cells, but mouse LIF cannot activate human cells. |
| Other Notes: | rhLeukemia Inhibitory Factor manufactured in the USA by InVitria. |
| Physical form: | 0.2 μm filtered solution at 500 μg/mL in buffered saline with 0.02% TWEEN20 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | >95% (SDS-PAGE) |
| Storage Temp. | −70°C |
| UNSPSC | 12352202 |