Lipopolysaccharides (rough strains) from Salmonella enterica serotype minnesota Re 595 (Re mutant)

SIGMA/L9764

Synonym: LPS

MDL Number: MFCD00131520
Product Type: Chemical

Catalog Number	PKG Qty.	Price


45-L9764-5MG	5 mg	$342.00 1/EA	Add To Favorites
45-L9764-10MG	10 mg	$642.00 1/EA	Add To Favorites

Lipopolysaccharides (LPS) are localized in the outer layer of the membrane and are, in noncapsulated strains, exposed on the cell surface. They contribute to the integrity of the outer membrane, and protect the cell against the action of bile salts and lipophilic antibiotics.

Properties
Descriptions
Safety Info.
Basic Data

biological source	Salmonella enterica (Serotype minnesota Re 595 mutant)
color	white to yellow cast
form	lyophilized powder
impurities	<3% Protein (Lowry)
Quality Level	200
shipped in	ambient
solubility	water: 0.90-1.10 mg/mL, hazy to turbid, colorless to faintly yellow
storage temp.	2-8°C

Application:	Charge and aggregation pattern govern the interaction of plasticins with LPS monolayers mimicking the external leaflet of the outer membrane of Gram-negative bacteria: This study examines how charge and aggregation patterns affect the interaction of plasticins with lipopolysaccharides (LPS) from rough strains like Salmonella enterica serotype Minnesota Re 595. The findings contribute to understanding the molecular interactions involved in microbial resistance mechanisms (Michel et al., 2015 ).
Biochem/physiol Actions:	Lipopolysaccharides (LPS) are localized in the outer layer of the membrane and are, in noncapsulated strains, exposed on the cell surface. They contribute to the integrity of the outer membrane, and protect the cell against the action of bile salts and lipophilic antibiotics.
General description:	This product is prepared from Salmonella Minnesota strain Re 595 (Re mutant) using a phenol-chloroform-petroleum ether extraction procedure. The source strain is ATCC 49284. A unique structure in the LPS of strain Re 595 was shown to induce secretion and aggregation in human platelets.
Other Notes:	To gain a comprehensive understanding of our extensive range of Lipopolysaccharides for your research, we encourage you to visit our Carbohydrates Category page.
Packaging:	5, 10 mg in glass bottle
Preparation Note:	Prepared by phenol-chloroform-petroleum ether extraction. The product is soluble in water (5 mg/ml) or cell culture medium (1 mg/ml) yielding a hazy, faint yellow solution. A more concentrated, though still hazy, solution (20 mg/ml) has been achieved in aqueous saline after vortexing and warming to 70-80 ^oC. Lipopolysaccharides are molecules that form micelles in every solvent. Hazy solutions are observed in water and phosphate buffered saline. Organic solvents do not give clearer solutions. Methanol yields a turbid suspension with floaters, while water yields a homogeneously hazy solution.

Symbol	GHS07
Signal word	Warning
Hazard statements	H302
Precautionary statements	P301 + P312 + P330
Hazard Codes	Xn
Risk Statements	22
RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point(F)	Not applicable
Flash Point(C)	Not applicable

Storage Temp.	2-8°C
UNSPSC	12352201

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