Lectin from Pseudomonas aeruginosa
SIGMA/L9895 - lyophilized powder
Synonym: PA-I
MDL Number: MFCD00165587
Product Type: Chemical
Agglutination
assay: Lectin from Pseudomonas aeruginosa (Cat. No.
L9895) at a concentration of ~1mg solid/mL was serially diluted (2-fold, top
to bottom of plate) and incubated with erythrocytes type: A (4% v/v RBC) to
calculate Minimum agglutination titer (MAT). Agglutination is defined as the
inability of cells to form a tight button. PBS was used as blank.
assay: Lectin from Pseudomonas aeruginosa (Cat. No.
L9895) at a concentration of ~1mg solid/mL was serially diluted (2-fold, top
to bottom of plate) and incubated with erythrocytes type: A (4% v/v RBC) to
calculate Minimum agglutination titer (MAT). Agglutination is defined as the
inability of cells to form a tight button. PBS was used as blank.
| form | lyophilized powder |
| potency | <15 μg/mL per mL agglutination activity |
| Quality Level | 200  |
| storage temp. | −20°C |
| Analysis Note: | Agglutination activity is expressed in μg/ml and is determined from serial dilutions of a 1 mg/ml solution using phosphate buffered saline, pH 7.0. This activity is the lowest concentration to agglutinate a 2% suspension of neuraminidase treated human blood group A erythrocytes after one hr incubation at 25 °C. |
| Analysis Note: | Highly purified. |
| Biochem/physiol Actions: | PA-I has a major affinity for |
| Packaging: | 1, 2 mg in glass bottle |
| Packaging: | Package size based on protein content. Vial content based on protein by biuret. |
| Physical form: | Lyophilized powder containing approx. 80% protein (biuret), balance primarily sodium citrate, plus traces of calcium chloride, magnesium chloride and manganese chloride |
| Preparation Note: | Purified by affinity chromatography using a modification of Gilboa-Garber.1 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352202 |