Anti-LRRK2 (C-terminal region) antibody produced in rabbit
SIGMA/L9918 - ~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution
Synonym: Anti-AURA17; Anti-Dardarin; Anti-Leucine-rich repeat protein kinase 2; Anti-PARK8; Anti-RIPK7; Anti-ROCO2
Product Type: Chemical
Enhanced Validation-By Recombinant Expression Antibody recognition: Anti-LRRK2 (C-terminal region) antibody produced in Rabbit (Cat. No. L9918) is confirmed by western blot in the cell sample that has been altered to overexpress the target protein-HEK-293T over expressing LRRK2 (Lane 1) while no staining is detected in the wild type sample-HEK-293T (Lane 2). The antibody was developed with Goat Anti-Rabbit IgG, Peroxidase conjugate (Cat. No. A0545) and a chemiluminescent substrate. Antibody dilution: 1:1,000.
Immunohistochemistry Staining of formalin-fixed, paraffin-embedded Human kidney sections with 30 μg/mL Rabbit Anti-LRRK2 (C-terminal region) (Cat. No. L9918) using biotin/ExtrAvidin®-Peroxidase.
Immunoblotting Cell and tissue lysates were separated on SDS-PAGE and probed with Anti-LRRK2 (C-terminal region) (Cat. No. L9918). The antibody was developed with Goat Anti-Rabbit IgG, Peroxidase conjugate (Cat. No. A0545) and a chemiluminescent substrate. Antibody dilution 1:1,000. Lanes 1. HEK 293T cells transfected with human LRRK2 2. HEK 293T cells untransfected 3. Mouse brain 4. Rat brain 5. Rat brain – Inhibition of antibody binding with LRRK2 immunizing peptide (human, 2079-2100) (10 μg/mL)
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1.0 mg/mL |
| conjugate | unconjugated |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation  |
| form | buffered aqueous solution |
| mol wt | antigen ~240 kDa |
| Quality Level | 200 |
| shipped in | dry ice |
| species reactivity | rat, mouse, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | western blot: 2-4 μg/mL using HEK-293T cells expressing human LRRK2, and using rat and mouse brain extracts (S2 fraction) |
| UniProt accession no. | Q5S007  |
| Application: | Anti-LRRK2 (C-terminal region) antibody is suitable for use in western blot (2-4 μg/mL using HEK-293T cells expressing human LRRK2, and using S2 fractions of rat and mouse brain extracts). |
| Biochem/physiol Actions: | Anti-LRRK2 (C-terminal region) antibody is specific for LRRK2 (approx. 240 kDa) in mice, rats and humans. |
| Biochem/physiol Actions: | LRRK2 is a μLtimeric kinase that also functions as a GTPase. LRRK2 activates a calcium/calmodulin-depend |
| Biochem/physiol Actions: | LRRK2 mRNA is expressed throughout the brain predominantly within regions of the basal ganglia that are associated with Parkinson′s disease. The presence of multiple protein interaction domains suggests that in addition to its kinase and GTPase activities, LRRK2 may serve as a scaffold protein for the assembly of multiprotein signaling complex. R1441C mutation has been shown to disrupt GTPase activity. G2019S and R1441C, both associated with increased kinase activity. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Leucine-rich repeat protein kinase 2 (LRRK2) comprises multiple domains, including a leucine-rich repeat (LRR) domain, a Roc (Ras of complex proteins) GTPase domain followed by a C-terminal of ROC (COR) domain, a Ser/Thr kinase domain and a C-terminal Trp-Asp-40 (WD40) domain. The N-terminal region (?900 amino acids) contains ankyrin (ANK) repeats. The LRRK2 homologue LRRK1 shares domain structure similarity with LRRK2. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |