QuickLink™ DNA Ligation Kit
SIGMA/LIG2 - joins blunt-end and sticky-end DNA fragments
Synonym: DNA Ligation Kit; Ligation Kit
EC Number: 232-770-0
Product Type: Chemical
Illustration of DNA ligation reaction.
| form | liquid |
| grade | Molecular Biology |
| Quality Level | 200  |
| shipped in | dry ice |
| storage temp. | −70°C |
| technique(s) | molecular cloning: suitable |
| usage | kit sufficient for 50 ligation reactions |
| Application: | QuickLink™ DNA Ligation Kit has been used for the ligation of pGL2-basic luciferase-reporter plasmid with vascular endothelial growth factor (VEGF) promoter sequence and for the ligation of NLR family caspase activating and recruitment domain (CARD)-containing protein 4 (Nlrc4) promoter with the luciferase plasmid vector pGL4.10-luc2 |
| Application: | The QuickLink™ DNA Ligation Kit is suitable for: • Joining blunt or cohesive-end fragments of DNA into a cloning vector • Recircularization of linear DNA • Formation of concatamers • dsDNA nick repair |
| Biochem/physiol Actions: | One of the most important steps in the cloning process is the ligation of linear DNA into a cloning vector. DNA ligations are performed by incubating DNA fragments with appropriately linearized cloning vectors in the presence of buffer, ATP, and DNA ligase. Many parameters affect ligations such as the relative ratio of insert to vector, the quality and type of the DNA ends, the temperature of ligation and the concentration of DNA. |
| Features and Benefits: | • Fast 5 minutes ligation • High ligation efficiency • Room temperature reactions – no cooling required • Perform bacterial transformation with the reaction mixture |
| General description: | Sigma’s DNA Ligation Kit contains all of the reagents necessary to perform DNA ligation reactions at room temperature using blunt or sticky ends. This kit replaces previous workflows requiring cooking steps and long incubations. Pre-made buffers allow for fast & easy setup. |
| Legal Information: | QuickLink is a trademark of Sigma-Aldrich Co. LLC |
| Other Notes: | Sufficient for 50 reactions: • 500uL 2X Ligation Buffer A (L9537) • 100uL 5X Ligation Buffer B (L9662) • 250 units T4 DNA Ligase (D2886) in 50% glycerol with 10 mM Tris-HCl (pH 7.5) 50 mM KCl, and 1 mM DTT |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −70°C |
| UNSPSC | 12352200 |