Minimum Essential Medium Eagle
SIGMA/M0268 - With Earle′s salts and L-glutamine, without sodium bicarbonate, powder, suitable for cell culture
Synonym: EMEM; MEM
Product Type: Chemical
| components | HEPES: no phenol red: yes Earle’s salts (5% CO2): yes NaHCO3: no L-glutamine: yes sodium pyruvate: no |
| form | powder |
| Quality Level | 500  |
| shipped in | ambient |
| storage temp. | 2-8°C |
| technique(s) | cell culture | mammalian: suitable |
| Application: | Minimum Essential Medium Eagle has been used: • in the isolation and culture of primary hepatocytes • to culture the human endometrial carcinoma cells Ishikawa (ATTC) and L-Wnt-3a cells (ATCC) that secrete Wnt family member 3a (Wnt-3a) ligand • to culture the AN3CA (endometrial cancer cell line) cells, and HEC1-B human (endometrial adenocarcinoma cell line) cells |
| Features and Benefits: | • Minimum Essential Medium Eagle (MEM) contains higher concentrations of amino acids to mimic the protein composition of cultured mammalian cells. • The formulation has been altered by optional elimination of calcium to enable the growth of cells in suspension culture. |
| General description: | Minimum Essential Medium Eagle (MEM) is one of the most widely used synthetic cell culture media. MEM has been used for culturing several cells grown in monolayers. It is commonly used to grow attached cell lines, such as fibroblasts, in the presence of fetal bovine sera (FBS), calf, or horse sera. |
| Other Notes: | Essentially the same as the original formula, except it is provided as a powder. |
| Preparation Note: | Formulated to contain 9.5 grams of powder per liter of medium. |
| Preparation Note: | Supplement with 2.2 g/L sodium bicarbonate. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352207 |