Anti-Mouse IgG (Fc specific) F(ab′)2 fragment antibody produced in goat
SIGMA/M0284 - 2.0 mg/mL, affinity isolated antibody, buffered aqueous solution
MDL Number: MFCD00162625
Product Type: Chemical
ELISA 10 μg/mL Anti-Mouse IgG (Fc specific) F(ab′)2 fragment antibody produced in Goat (Cat. No. M0284), followed by 1:10,000 Anti-Goat IgG (whole molecule)-Alkaline Phosphatase antibody produced in Rabbit (Cat. No. A4187), was probed to detect Mouse IgG by indirect ELISA. Reactivity is observed with Mouse IgG and Mouse FC but not with Mouse Fab, Human IgG, Human IgA, Human IgM, Bovine IgG, Bovine IgM and Horse IgG.
| antibody form | affinity isolated antibody |
| antibody product type | secondary antibodies |
| biological source | goat |
| clone | polyclonal |
| concentration | 2.0 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| Quality Level | 200  |
| shipped in | dry ice |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect ELISA: suitable |
| Application: | Anti-Mouse IgG (Fc specific) F(ab′)2 fragment antibody produced in goat has been used in CD117/Sca-1 fluorescence-activated cell sorting (FACS) analysis and to detect bound factor H in factor H binding assay. |
| Biochem/physiol Actions: | Immunoglobulin G (IgG) participates in hypersensitivity type II and type III reactions. IgG helps in opsonization, complement fixation and antibody dependent cell mediated cytotoxicity. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Immunoglobulin G (IgG) belongs to the immunoglobulin family and is a widely expressed serum antibody. An immunoglobulin has two heavy chains and two light chains connected by a disulfide bond.It mainly helps in immune defense. It is a glycoprotein.IgG is a major class of immunoglobulin.Mouse consists of five immunoglobulin classes- IgM, IgG, IgA, IgD and IgE. Mouse IgG is further divided into five classes- IgG1, IgG2a, IgG2b and IgG3. |
| General description: | Immunoglobulin G (IgG) is a glycoprotein antibody that regulates immune responses such as phagocytosis and is also involved in the development of autoimmune diseases . Mouse IgGs have four distinct isotypes, namely, IgG1, IgG2a, IgG2b, and IgG3. IgG1 regulates complement fixation in mice . |
| Immunogen: | Purified mouse IgG |
| Other Notes: | Antibody adsorbed with bovine, equine and human serum proteins |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 0.05% sodium azide. |
| Preparation Note: | Adsorbed to reduce background with bovine, equine, or human samples. Useful when trying to avoid background staining due to the presence of Fc receptors. |
| Hazard Codes | Xn |
| Risk Statements | 21/22 |
| Safety Statements | 36/37-60 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |