MDM2, GST-tagged human
SIGMA/M0321 - recombinant, expressed in E. coli, buffered aqueous glycerol solution, ≥75% (SDS-PAGE)
Product Type: Chemical
This image is provided for informative purposes only and does not represent an actual product label. It should not be used as a substitute for official product labeling.
| assay | ≥75% (SDS-PAGE) |
| biological source | human |
| description | contains an N-terminal GST tag |
| form | buffered aqueous glycerol solution |
| mol wt | ~84 kDa |
| Quality Level | 200  |
| recombinant | expressed in E. coli |
| shipped in | dry ice |
| storage temp. | −70°C |
| UniProt accession no. | Q00987  |
| Biochem/physiol Actions: | Mdm2 functions as an E3 ubiquitin ligase and an inhibitor of p53 transcriptional activation. |
| Biochem/physiol Actions: | MDM2 is a negative regulator of the p53 tumor suppressor. |
| Biochem/physiol Actions: | Murine double minute 2 (MDM2) is a chief regulator of the p53 tumor suppressor protein. It is a multifunctional oncoprotein that contributes to both p53-dependent and -independent roles in oncogenesis. MDM2 has various p53-independent functions that contribute to its activity as an oncogene. |
| Biochem/physiol Actions: | Overexpression of Murine double minute 2 (MDM2) is observed in various cancer like lung, gastric, colorectal, hepatocellular, breast, endometrial, and prostate. A common single nucleotide polymorphism is observed at 309 amino acid (from T to G) increases the susceptibility to cancer. |
| General description: | Murine double minute 2 (MDM2) encoding gene is localized at 12q15 in the human chromosome. It is a multidomain nuclear phosphoprotein. It possesses really interesting new gene (RING) finger domain and its function and structural integrity is required for the action of MDM2. It also has central acidic domain. |
| Packaging: | 10 μg in glass bottle |
| Physical form: | Solution in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF and 25% glycerol. |
| Preparation Note: | Supplied as a truncated version (202-end) with an N-terminal GST tag |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥75% (SDS-PAGE) |
| Storage Temp. | −70°C |
| UNSPSC | 12352202 |