Medium 199
SIGMA/M0393 - With Hanks′ salts and L-glutamine, without sodium bicarbonate, powder, suitable for cell culture
Synonym: M199 Medium; TCM 199
Product Type: Chemical
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| components | HEPES: no Hanks’ salts (2% CO2): yes sodium pyruvate: no NaHCO3: no L-glutamine: yes phenol red: yes |
| form | powder |
| Quality Level | 500  |
| shipped in | ambient |
| storage temp. | 2-8°C |
| technique(s) | cell culture | mammalian: suitable |
| Application: | Medium 199 has been used: • to test and determine the pH sensitivity of the sensors in a complex environment • as a culture medium during oocyte harvesting • as a component of Leishmania donovani (LdBOB) promastigote media for culturing L. donovani • to culture visceral adipose tissue explants |
| General description: | Medium 199 is a combination of vitamins, amino acids, and other factors that were originally developed as a completely defined media formulation for the culture of primary explants. This medium, when properly supplemented has broad applicability, particularly for non-transformed cells. Medium 199 is widely used for vaccine production, in vitro cultivation of primary pancreatic explants, and lens tissues. |
| Preparation Note: | Formulated to contain 10.6 grams of powder per liter of medium. |
| Preparation Note: | Supplement with 0.35 g/L sodium bicarbonate. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352207 |