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6-Fluorescein Phosphoramidite

SIGMA/M041130 - configured for ABI

Synonym: 6-FAM-phosphoramidite

CAS Number: 204697-37-0
Empirical Formula (Hill Notation): C46H58N3O10P
Molecular Weight: 843.94
MDL Number: MFCD01940925
Linear Formula: C46H58N3O10P
Product Type: Chemical
Catalog Number PKG Qty. Price Quantity
45-M041130-0.1G 0.1 g
 $143.00
1/EA		 Add To Favorites
45-M041130-0.25G 0.25 g
 $602.00
1/EA		 Add To Favorites

 

λ conforms (UV/VIS Identity)
assay ≥97% (31P-NMR)
  ≥97.0% (reversed phase HPLC)
color solid white to off-white
compatibility configured for ABI
InChI 1S/C46H58N3O10P/c1-29(2)49(30(3)4)60(55-25-15-22-47)54-24-14-12-11-13-23-48-40(50)31-16-19-34-37(26-31)46(59-41(34)51)35-20-17-32(56-42(52)44(5,6)7)27-38(35)58-39-28-33(18-21-36(39)46)57-43(53)45(8,9)10/h16-21,26-30H,11-15,23-25H2,1-10H3,(H,48,50)
InChI key MGPYJVWEJNTXLC-UHFFFAOYSA-N
product line Proligo Reagents
Quality Level 200 
SMILES string CC(C)N(C(C)C)P(OCCCCCCNC(=O)c1ccc2C(=O)OC3(c4ccc(OC(=O)C(C)(C)C)cc4Oc5cc(OC(=O)C(C)(C)C)ccc35)c2c1)OCCC#N
solubility acetonitrile: 0.1 M, clear, colorless to faintly yellow (liquid)
storage temp. −20°C
suitability conforms to structure for H-NMR
type for DNA synthesis
General description: Phosphoramidite technology is ideally suited for the modification ofsynthetic oligonucleotides with covalently attached reporter moieties,linkers, spacers, or haptens. Sigma-Aldrich provides popular reporters andlinkers ready to use for DNA/RNA synthesis machines such as β-cyanoethylphosphoramidites. 6-Fluorescein Phosphoramidite is one such label whose absorbance maxima is 496 nm and emission maxima is 520 nm.Key Features of 6-Fluorescein Phosphoramidite:

• 6-Fluorescein Phosphoramidite consists of one isomer derived from6-carboxy fluorescein
•  Couples to the 5′-end of the oligonucleotide using standard synthesisprotocols
•  Protecting groups on the fluorescein moiety are removed under standardconditions of cleavage and deprotection with concentrated ammonia
• A detritylation step is not required since the fluorescein phosphoramiditedoes not contain a DMT-group
• The labeled oligonucleotide is ready for use in most applications after theevaporation of ammonia. Standard procedures can be used if additionalpurification is required.
• Lot-to-lot consistent purity and performance
• Manufactured under a certified ISO 9001 quality system6-Fluorescein Phosphoramidite is configured for ABI Synthesizers.
RIDADR NONH for all modes of transport
WGK Germany WGK 3
Flash Point(F) Not applicable
Flash Point(C) Not applicable
Purity ≥97% (31P-NMR); ≥97.0% (reversed phase HPLC)
Storage Temp. −20°C
UNSPSC 41116105

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