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Biotin Phosphoramidite

SIGMA/M042030 - configured for ABI

Synonym: Biotin amidite

CAS Number: 1217500-22-5
Empirical Formula (Hill Notation): C46H64N5O8PS
Molecular Weight: 878.07
MDL Number: MFCD00676535
Linear Formula: C46H64N5O8PS
Product Type: Chemical
Catalog Number PKG Qty. Price Quantity
45-M042030-0.1G 0.1 g
 $163.00
1/EA		 Add To Favorites
45-M042030-0.25G 0.25 g
 $317.00
1/EA		 Add To Favorites

 

assay ≥96% (31P-NMR)
compatibility configured for ABI
InChI 1S/C46H64N5O8PS/c1-34(2)51(35(3)4)60(58-27-12-25-47)59-32-31-57-30-29-56-28-26-48-43(52)16-11-10-15-42-44-41(33-61-42)50(45(53)49-44)46(36-13-8-7-9-14-36,37-17-21-39(54-5)22-18-37)38-19-23-40(55-6)24-20-38/h7-9,13-14,17-24,34-35,41-42,44H,10-12,15-16,26-33H2,1-6H3,(H,48,52)(H,49,53)/t41-,42-,44-,60?/m0/s1
InChI key WSDQYLGSIUJJRU-ZMKGMYJVSA-N
product line Proligo Reagents
Quality Level 200 
SMILES string COc1ccc(cc1)C(N2C3CS[C@@H](CCCCC(=O)NCCOCCOCCOP(OCCC#N)N(C(C)C)C(C)C)C3NC2=O)(c4ccccc4)c5ccc(OC)cc5
storage temp. −20°C
suitability conforms to structure for H-NMR
General description: Biotin phosphoramidite is a reagent used to directly incorporate a biotin label at the 5′ hydroxy group of the terminal nucleoside base through a p-aminophenethyl spacer group during oligonucleotide synthesis. Biotin-labelled oligonucleotides have become extremely popular in DNAcapture and detection applications, due to their very high-sensitivity and strong, specific binding affinity with avidin and streptavidin proteins. Biotinbased detection methods and are widely used by researchers. Biotin labelling is compatible with PCR and most hybridization techniques.

Key Features of Biotin Phosphoramidite

• Behaves like any standard phosphoramidite on the DNA synthesizer
•  Readily soluble in acetonitrile
• Biotin compound has a dimethoxytrityl (DMT) group on the ring structure,which allows coupling-yield determination and trityl-selective purification
• A long, water-compatible linker arm ensures maximum sensitivity
•  Provides a coupling efficiency of at least 95%The product is configured for ABI™ Synthesizers
Legal Information: ABI is a trademark of Applera Corporation or its subsidiaries in the US and/or certain other countries
RIDADR NONH for all modes of transport
WGK Germany WGK 3
Flash Point(F) Not applicable
Flash Point(C) Not applicable
Purity ≥96% (31P-NMR)
Storage Temp. −20°C
UNSPSC 41116105

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