Biotin Phosphoramidite

SIGMA/M042080 - configured for PerkinElmer, configured for Polygen

Synonym: Biotin amidite

CAS Number: 1217500-22-5
Empirical Formula (Hill Notation): C₄₆H₆₄N₅O₈PS
Molecular Weight: 878.07
MDL Number: MFCD00676535
Linear Formula: C₄₆H₆₄N₅O₈PS
Product Type: Chemical

Catalog Number	PKG Qty.	Price	Quantity


45-M042080-0.1G	0.1 g	$163.00 1/EA		Add To Favorites
45-M042080-0.25G	0.25 g	$327.00 1/EA		Add To Favorites

Properties
Descriptions
Safety Info.
Basic Data

assay	≥96% (³¹P-NMR)
compatibility	configured for PerkinElmer
	configured for Polygen
InChI	1S/C46H64N5O8PS/c1-34(2)51(35(3)4)60(58-27-12-25-47)59-32-31-57-30-29-56-28-26-48-43(52)16-11-10-15-42-44-41(33-61-42)50(45(53)49-44)46(36-13-8-7-9-14-36,37-17-21-39(54-5)22-18-37)38-19-23-40(55-6)24-20-38/h7-9,13-14,17-24,34-35,41-42,44H,10-12,15-16,26-33H2,1-6H3,(H,48,52)(H,49,53)/t41-,42-,44-,60?/m0/s1
InChI key	WSDQYLGSIUJJRU-ZMKGMYJVSA-N
product line	Proligo Reagents
Quality Level	200
SMILES string	COc1ccc(cc1)C(N2C3CS[C@@H](CCCCC(=O)NCCOCCOCCOP(OCCC#N)N(C(C)C)C(C)C)C3NC2=O)(c4ccccc4)c5ccc(OC)cc5
storage temp.	−20°C
suitability	conforms to structure for H-NMR

Application:	Biotin Phosphoramidite is suitable for the synthesis of 5′-biotinylated oligonucleotides whose applications are seen in ligase-mediated gene detection, direct dideoxy sequencing following PCR, and non-radioactive sequencing of DNA.
General description:	Biotin phosphoramidite is a reagent used to directly incorporate a biotin label at the 5′ hydroxy group of the terminal nucleoside base through a p-aminophenethyl spacer group during oligonucleotide synthesis. The photocleavable biotin phosphoramidite reagent is compatible with automated DNA/RNA synthesizers using phosphoramidite chemistry. The length of the p-aminophenethyl spacer group allows streptavidin affinity purification of the oligonucleotide by reverse-phase HPLC. Biotinlabeling is compatible with PCR and most hybridization techniques.Key Features of Biotin Phosphoramidite• Behaves like any standard phosphoramidite on the DNA synthesizer • Readily soluble in acetonitrile • Biotin compound has a dimethoxytrityl (DMT) group on the ring structure,which allows coupling-yield determination and trityl-selective purification• A long, water-compatible linker arm ensures maximum sensitivity • Provides a coupling efficiency of at least 95%The product is configured for Expedite^™ and PolyGen^® Synthesizers.
Legal Information:	Expedite is a trademark of Applera Corporation or its subsidiaries in the US and/or certain other countries
Legal Information:	PolyGen is a registered trademark of PolyGen GmbH.

RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point(F)	Not applicable
Flash Point(C)	Not applicable

Purity	≥96% (³¹P-NMR)
Storage Temp.	−20°C
UNSPSC	41116105

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