Mitomycin C from Streptomyces caespitosus
SIGMA/M0440 - meets USP testing specifications
Synonym: Mitomycin
CAS Number: 50-07-7
Empirical Formula (Hill Notation): C15H18N4O5
Molecular Weight: 334.33
EC Number: 200-008-6
MDL Number: MFCD00078109
Linear Formula: C15H18N4O5
Product Type: Chemical
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: M0440-25MG
| antibiotic activity spectrum | Gram-negative bacteria |
| Gram-positive bacteria | |
| biological source | Streptomyces caespitosus |
| color | blue-violet |
| InChI | 1S/C15H18N4O5/c1-5-9(16)1 |
| InChI key | NWIBSHFKIJFRCO-WUDYKRTCSA |
| mode of action | DNA synthesis | interferes |
| Quality Level | 200  |
| SMILES string | [H][C@]12CN3C4=C([C@@H](C |
| solubility | H2O: 40 mg/mL, clear to very slightly hazy, colorless |
| H2O: soluble | |
| storage temp. | 2-8°C |
| Application: | Mitomycin C is produced by a strain of actinomyces, Streptomyces caespitosus. It contains three anticancer moieties, quinine, urethane, and aziridine groups. It is used to generate mitotically inactive feeder cells in cell culture systems, such as the mitotically inactive fibroblasts used in embryonic stem cell systems. |
| Biochem/physiol Actions: | Mode of Action: This product is an alkylating agent that specifically targets the guanine nucleoside sequence 5′-CpG-3′. It inhibits DNA synthesis by covalently reacting with DNA, forming crosslinks between complementary strands of DNA. This interaction prevents separation of complementary DNA strands, inhibiting DNA replication. Antimicrobial Spectrum: Mitomycin C has strong antitumor activity, especially against Ehrlich ascites tumor cells, and strong bactericidal action against gram-positive and gram-negative bacteria. |
| Disclaimer: | Stock solutions should be filter sterilized and stored at 2-8 °C in the dark. Solutions at pH 6-9 can be stored at 0-5 °C for up to a week, but if a precipitate forms, a fresh solution should be prepared - the precipitated solution has been proven toxic to cells. |
| General description: | Chemical structure: aziridine |
| Packaging: | 5, 25 mg in glass bottle |
| Preparation Note: | Mitomycin C is soluble in water at .5 mg/mL, with a pH of 6.0-7.5. It undergoes rapid degradation in acidic solutions with pH<6, and is mostly likely to retain activity in solutions with a pH between 6-9. |
| Symbol |   GHS06,GHS08 |
| Signal word | Danger |
| Hazard statements | H300 - H351 |
| Precautionary statements | P201 - P301 + P310 + P330 |
| Hazard Codes | T |
| Risk Statements | 25-40 |
| Safety Statements | 28-36/37-45 |
| RIDADR | UN 3462 6.1 / PGII |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |