Anti-MDMX antibody, Mouse monoclonal
SIGMA/M0445 - clone MDMX-82, purified from hybridoma cell culture
Synonym: Anti-HDMX; Anti-MDM4; Anti-p53 Binding Protein
MDL Number: MFCD00282354
Product Type: Chemical
Enhanced Validation-By Recombinant Expression Antibody recognition: Monoclonal Anti-MDMX antibody produced in Mouse, Clone: MDMX-82 (Cat. No. M0445) is confirmed by western blot in the cell sample that has been altered to overexpress the target protein-HEK-293T over expressing MDMX (Lanes 1-3) while no staining is detected in the wild type sample-HEK-293T (Lanes 4-6). The antibody was developed with 1:5,000 Anti-Mouse IgG (Fab specific)-Peroxidase antibody produced in Goat (Cat. No. A2304) and a chemiluminescent substrate. Antibody dilutions 1. 2 μg/mL antibody 2. 1 μg/mL antibody 3. Negative Control 4. Untransfected, 2 μg/mL 5. Untransfected, 1 μg/mL 6. Untransfected, Negative Control
Immunoblotting Whole extract of human HEK-293T cells overexpressing human MDMX was separated on SDS-PAGE and probed with Monoclonal Anti-MDMX Clone: MDMX-82 (Cat. No. M0445). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1. 0.5 μg/mL antibody 2. Negative control: without 1st antibody
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | MDMX-82, monoclonal |
| concentration | ~2 mg/mL |
| conjugate | unconjugated |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation  |
| form | buffered aqueous solution |
| isotype | IgG1 |
| mol wt | antigen ~80 kDa |
| Quality Level | 200 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunocytochemistry: suitable |
| immunoprecipitation (IP): suitable | |
| microarray: suitable | |
| western blot: 1-2 μg/mL using total cell extract of HEK 293T cells transfected with human MDMX | |
| UniProt accession no. | O15151  |
| Application: | Monoclonal Anti-MDMX antibody is suitable for use in immunocytochemistry, immunoprecipitation, immunoblot (approx. 80 kDa), microarray, and ELISA. The product may also be used for western blot and for assessing ectopic expression of MDMX (using a conc. of 1-2 μg/mL in total cell extract of HEK 293T cells transfected with human MDMX). |
| Biochem/physiol Actions: | MDMX is a structural homolog of MDM2 and inhibits the tumor suppressor, p53. MDMX also prevents MDM2-mediated degradation of p53, and subsequently regulates cellular levels of p53. MDMX also modulates cell growth and survival. Thus, MDMX can be useful as a therapeutic target in cancers, especially in tumors where native p53 functions can be restored . Monoclonal Anti-MDMX antibody is specific for human MDMX. |
| Biochem/physiol Actions: | Monoclonal Anti-MDMX recognizes human MDMX. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Double minute 4 protein or MDMX is a nuclear protein. The gene encoding it is localized on human chromosome 1q32.1. |
| General description: | Monoclonal Anti-MDMDX (mouse IgG1 isotype) is derived from the hybridoma MDMX-82 produced by the fusion of mouse myeloma cells (NS1 cells) and splenocytes from BALB/c mice. MDMX belongs to the MDM family. The MDMX/ mouse double minute 4 (MDM4) gene encode a 490 amino acid protein. MDMX comprises a putative nuclear localization signal, a really interesting new gene (RING) finger domain in the C terminal and a p53-binding domain in the N terminal. MDMX has significant structural similarity to MDM2 E3 ubiquitin ligase. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| Preparation Note: | For continuous use, store at 2–8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |