Mycoplasma Broth
SIGMA/M0535 - powder, suitable for microbiology
Synonym: Mycoplasma media
Product Type: Chemical
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: M0535-250G
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: M0535-500G
| application(s) | microbiology |
| form | powder |
| Quality Level | 200  |
| solubility | H2O: 25.5 g/L (Autoclave at 121°C, 15 psi for 15 minutes. Store prepared medium at 0-5°C.) |
| technique(s) | microbiological culture: suitable |
| Application: | Mycoplasma Broth has been used for isolation and cultivation of mycoplasma. It may also be used to grow Porphyromonas gingivalis under anerobic conditions. |
| General description: | Mycoplasma Broth is a basal medium which, after enrichment with yeast extract, horse serum, and antibiotics, will support the growth of Mycoplasma sp. The components contained in this basal medium do not exhibit inhibitory or toxic effects when used to culture mycoplasma. A special mineral supplement has been added to improve the growth and colony characteristics of the organism. |
| Other Notes: | Powder contains (g/L): 10.0 bacterial peptone, 10.0 LAB-LEMCO powder, 5.0 sodium chloride, 0.5 mineral supplement. |
| Other Notes: | The product is intended for laboratory use only. |
| Packaging: | 250, 500 g in poly bottle |
| Preparation Note: | Basal Medium: 1. Suspend 25.5 g of powdered medium in 1L of distilled water. Stir until the powder dissolves. 2. Adjust the pH of the solution to 7.8 ± 0.2. 3. Autoclave at 121 °C and 15 psi for 15 minutes to sterilize. Cool to 50 °C and add sterile supplements. |
| Preparation Note: | Store dry powdered medium at room temperature and prepare medium at 2-8 °C. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| UNSPSC | 41106200 |