Phosphate-ON Phosphoramidite
SIGMA/M061040 - configured for MerMade
Synonym: Phosphate-ON amidite
Empirical Formula (Hill Notation): C49H62N7O11P
Molecular Weight: 956.03
MDL Number: MFCD12912424
Linear Formula: C49H62N7O11P
Product Type: Chemical
| assay | ≥98% (31P-NMR) |
| ≥98.0% (reversed phase HPLC) | |
| compatibility | configured for MerMade |
| InChI | 1S/C49H62N7O11P/c1-33(2)5 |
| InChI key | MCGIOYZUTKDSIL-NNUNARBDSA |
| product line | Proligo Reagents |
| Quality Level | 200  |
| SMILES string | COc1ccc(cc1)C(OC[C@H]2O[C |
| storage temp. | −20°C |
| type | for DNA synthesis |
| Application: | The application of Phosphate-ON Phosphoramidite is fully compatible with standard cleavage and deprotection procedures at the end of oligonucleotide synthesis. Phosphate-ON phosphoramidite comprises a thymidine nucleoside, which is removed from the oligonucleotide during the cleavage and deprotection process. |
| General description: | Phosphate-ON phosphoramidite is a chemical phosphorylation reagent that can be employed to introduce a phosphate group at the 5′ terminus of an oligonucleotide. Chemical phosphorylation is a cost-effective alternative to enzymatic phosphorylation methods, allowing the introduction of terminal phosphate groups in high yields, at any desired scale. We offer Phosphate-ON phosphoramidite in powder form, enabling the dissolution of the reagent in acetonitrile to be easily monitored. |
| Legal Information: | This product is covered under US Patent Number 7,276,598 |
| Other Notes: | The key features of Phosphate-ON Phosphoramidite are as follows:• Powder format enables dissolution to be easily monitored and facilitatesproduct handling • Fully compatible with standard phosphoramidite reagents and synthesisconditions • Compatible with dG(dmf) fast deprotection chemistry: the oligonucleotidecan be cleaved from the support and deprotected in concentratedammonia at 55 °C in only 2 hours • Comprises a DMT-group for trityl monitoring • Dissolves easily in acetonitrile to a 0.1 M solution • High coupling efficiency of ≥98.0% under standard DNA phosphoramiditecoupling conditions • Cleavage of both phosphate protective groups through a β-eliminationprocess |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥98% (31P-NMR); ≥98.0% (reversed phase HPLC) |
| Storage Temp. | −20°C |
| UNSPSC | 41116105 |