Minimum Essential Medium Eagle
SIGMA/M0644 - Alpha Modification, with L-glutamine, ribonucleosides and deoxyribonucleosides, without sodium bicarbonate, powder, suitable for cell culture
Synonym: MEM
Product Type: Chemical
| components | NaHCO3: no L-glutamine: yes phenol red: yes HEPES: no sodium pyruvate: yes |
| form | powder |
| Quality Level | 500  |
| shipped in | ambient |
| storage temp. | 2-8°C |
| technique(s) | cell culture | mammalian: suitable |
| Application: | Minimum essential medium Eagle has been used to: • maintain natural killer (NK) cell lines • culture Leishmania donovani strains • culture epidermal neural crest stem cells (EPI-NCSCs) from the bulge of rat hair follicles |
| General description: | Minimum Essential Medium (MEM) is a synthetic cell culture media developed by Harry Eagle. This medium has higher concentrations of amino acids so the medium more closely approximates the protein composition of cultured mammalian cells. Optional supplementation of non-essential amino acids to this formulation that incorporates either Hanks′ or Earle′s salts has broadened the usage of this medium. The α modification of MEM with Earle′s balanced salts also known as αMEM, contains non-essential amino acids, sodium pyruvate, and additional vitamins. |
| Preparation Note: | Formulated to contain 10.1 grams of powder per liter of medium. |
| Preparation Note: | Supplement with 2.2 g/L sodium bicarbonate. |
| Symbol |  GHS07 |
| Signal word | Warning |
| Hazard statements | H317 |
| Precautionary statements | P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352207 |