Medium 199
SIGMA/M0650 - 10 ×, With Earle′s salts, without L-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture
Synonym: Cell growth medium; M199 Medium; TCM 199
Product Type: Chemical
| components | NaHCO3: no phenol red: yes sodium pyruvate: no HEPES: no Earle’s salts (5% CO2): yes L-glutamine: no |
| concentration | 10 × |
| form | liquid |
| impurities | endotoxin, tested |
| Quality Level | 400  |
| shipped in | ambient |
| sterility | sterile-filtered |
| storage temp. | 2-8°C |
| technique(s) | cell culture | mammalian: suitable |
| Application: | Medium 199 has been used: • to culture human mesothelial cells • as a component of the holding medium for the collection of embryos from pluriparous Boer goat • in the preparation of spheroid-embedded collagen matrices |
| General description: | Medium 199 is a combination of vitamins, amino acids, and other factors that were originally developed as a completely defined media formulation for the culture of primary explants. This medium, when properly supplemented has broad applicability, particularly for non-transformed cells. Medium 199 is widely used for vaccine production, in vitro cultivation of primary pancreatic explants, and lens tissues. |
| Preparation Note: | Supplement with 0.1 g/L |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352207 |